Now showing items 1-20 of 1478

    • Understanding the Language of Information Literacy

      Orgeron, Jean-Paul (Elsevier, 2018-01)
      Understanding the language of information literacy is necessary for the effective use of library resources. The results of a recent study indicate that undergraduate students lack such an understanding, and the authors recommend that librarians, working with faculty, reassess information literacy terms. This article examines what is involved in reassessing these terms by drawing on several ideas from the philosophy of language, which provides a foundation for grasping the semantic challenges librarians face in educating users. Any reassessment of information literacy terms should recognize their ordinary and specialized use and aim for the holistic expression of core concepts, however complex they may be.
    • The state of Crumhorn Lake, 2021 and a plan for the management of Crumhorn Lake

      Stich, Daniel S.; Pfuhler, David Mathias (SUNY Oneonta, 2021)
    • Identification of Fireflies (Coleoptera: Lampyridae) at Thayer Farm, Otsego County, NY

      Heilveil, Jeffrey S.; Cassata, Samantha Angela (SUNY Oneonta, 2019)
    • Doublespeak: Louisa Jacobs, the American Equal Rights Association, and Complicating Racism in the Early US Women’s Suffrage Movement,

      Goodier, Susan (Cornell Press, 2021)
      Members of the U.S. women’s suffrage movement, usually noted as being from the 1840s to the Nineteenth Amendment in 1920, faced many struggles related to race from the outset. Periods of close collaboration between Black and white activists have been punctuated by longer periods with virtually no cooperation between them. Turning our attention to Lou- isa Jacobs, the daughter of the once-enslaved Harriet Jacobs (author of Incidents in the Life of a Slave Girl), helps us unpack racial cooperation—and the lack thereof—in the years immediately following the Civil War.
    • Short‐term responses of freshwater mussels to floods in a southwestern U.S.A. river estimated using mark–recapture sampling

      Stich, Daniel S.; Sotola, V. A.; Sullivan, K. T.; Littrell, B. M.; Martin, N. H.; Bonner, T. H. (Wiley, 2021)
      Floods can directly affect riverine organisms by displacing them, and population‐level responses to floods can vary depending on flood magnitude and organism mobility. Benthic organisms can resist displacement until substrates become unstable, whereas mobile organisms are generally more resilient. Freshwater mussels are benthic organisms with low mobility, and there is limited research on their population‐level responses to floods. This study provides novel insights to population‐level responses of mussels to large floods (>500 m3/s). Population dynamics (i.e. abundance, survival, and site fidelity) and sampling efficiency (i.e. detection probability) were estimated in a robust design framework for four freshwater mussel species (Cyclonaias petrina, Cyclonaias pustulosa, Amblema plicata, and Tritogonia verrucosa) from 2017 to 2019 at two sites (upper and lower sites) within riffle habitats in the Colorado River, Texas, U.S.A. Individuals of each species were affixed with shellfish tags, with C. petrina and C. pustulosa individuals also being affixed with passive integrated transponder tags. Changes in population dynamics related to the flood event at each site were directly tested. During sampling, a major flood occurred at each of the two study sites; the floods differed in magnitude but were in the 99th percentile of historical flows at their respective gages. There were site‐ and species‐specific differences in estimated abundances, survival, and site fidelity during periods with the floods. Estimated abundances of C. petrina, C. pustulosa, and T. verrucosa were reduced 40–78% by the lesser flood magnitude (1,283 m3/s) at the upper site. Estimated abundances of C. petrina, C. pustulosa, and A. plicata were reduced 93–95% by the greater flood magnitude (4,332 m3/s) at the lower site. There was a reduction in survival of C. petrina at the upper site, while initially high survival at the lower site was reduced during the interval with the flood for all species. Finally, there was a reduction in site fidelity of C. pustulosa at the lower site. Floods reduced the abundance of all species within riffle habitats at the two sites. Large floods, therefore, affect population dynamics of mussels, but the fate of the displaced mussels is unknown, and with limited inference, reach‐scale effects are unknown. This study adds to the growing body of knowledge about responses of aquatic organisms to large floods, although quantification of recolonisation and fate of displaced mussels are needed to fully understand long‐term effects of large floods on mussel communities.
    • The state of Lake Forest and Lake Allure, NY, 2020 and a plan for the management of lakes Forest and Allure

      Stich, Daniel S.; Carey, Samantha (SUNY Oneonta, 2020)
      Lake Forest and Lake Allure are two private waterbodies situated in the “Forever Wild” Adirondack Park. The impoundments were formed in the early 1900s when dams were constructed on Stewart Brook and Stewart Creek by Earl Woodward. Soon after, the Northwoods Lake Association (NWLA) was founded to serve, protect and manage these resources. NWLA members serve as vested lake stewards who are invested in the care of these lakes. These impoundments are cherished by residents, visitors and the Northwoods Lake Association, who strive to both manage and protect the resources. The State of Lake Forest and Lake Allure, NY and Comprehensive Lake Management Plan for Lakes Forest and Allure was created to provide the above parties with the information, tools, resources and recommendations to help preserve and protect the lakes for future generations.
    • Role of RPTPzeta/phosphacan in the neural extracellular matrix

      Matthews, Rick; Eill, Geoffrey (2020)
      The mammalian brain is the most structurally and functionally complex system in biology. In order to carry out diverse functions such as thought and cognition, neurons in the brain must properly differentiate, make millions of functional interconnections, and incredibly be able to maintain those interconnections while retaining plasticity and the ability to learn throughout its lifetime. In order for neurons to carry out these complex functions, they must have an intricate relationshipwith their extracellular environment, which provides numerous molecules, such as growth factors and neurotransmitters, and physical cues to initiate critical downstream signaling cascades. In the central nervous system (CNS), the neural extracellular matrix (ECM) largely organizes this extracellular environment, and as such, is implicated in a multitude of neuronal functions. Not only can it serve as a physical barrier, the neural ECM is shown to regulate neuronal cell differentiation, migration, synaptogenesis, and maintain the mature state by restricting plasticity and neurite outgrowth. Of particular import, a subset of the neural ECM, the perineuronal net (PNN), is implicated to regulate neuronal plasticity in the CNS. PNNs were historically believed to be critical for restricting experience dependent plasticity in the brain but more recently shown to regulate several forms of learning and memory, in addition to multiple neurological diseases. However, despite these implicated functions, PNNs comprise only a fraction of the total ECM in the CNS. A majority of the neural ECM is derived from the diffuse ECM, a structure that is ubiquitously expressed throughout the CNS. As the diffuse ECM is very similar in molecular composition and structure to PNNs, it is difficult to specifically target PNNs for functional and mechanistic studies.As such, a better toolset is needed to differentiate the contributions ivbetween PNNs and the diffuse ECM in regulating plasticity and learning and memory. To develop thistoolset, there is a great need to better understand PNN molecular composition and structure. Therefore, the purpose of this work was to provide critical insight into the molecular composition and structure of PNNs soas to better understand its function in the CNS. In Chapter 2, using multiple ECM genetic knockout models, we show compelling evidence of an additional PNN anchor that importantlylays the groundworkforfuture functional studies. Specifically, we suggestchondroitin sulfate proteoglycan (CSPG) phosphacan, secreted isoform of receptor protein tyrosine phosphatase zeta (RPTPζ), iscritical for PNN structure as it partially anchors PNNs to the neuronal surface through cooperation with tenascin-R. Additionally, as the neural ECM is involved in numerous neurological diseases, in Chapter 3, we investigated the function of major ECM component, RPTPζ, in a group of O-mannosyl related congenital muscular dystrophy with associated brain abnormalities (CMD). Our data suggest a possible role of RPTPζ in proper cortical lamination in a CMD mouse model. Interestingly, we found evidence of a novel O-mannosyl substrate in the developing brain that could critically contribute to the underlying deficits of CMD. In conclusion, the neural ECM, once previously disregarded in the field, is becoming a novel source to understand mature mammalian brain function and disease, but more work is needed to better differentiate the specific roles of its substructures.

      King, Christine; Endy, Timothy; Barbachano-Guerrero, Arturo (2020)
      Dengue virus (DENV) causes an estimated 390 million infections worldwide annually, with severe forms of disease marked by vascular leakage and an over reactive inflammatory response. Endothelial cells (EC) are directly responsible for vascular homeostasis and are highly responsive to circulating mediators but are not commonly infected. Mast cells (MC) are potent cells of the innate immune system that play an important role in EC biology and inflammatory responses. DENV encodes 10 proteins; with only one, the non-structural protein 1 (NS1), secreted from infected cells and accumulating in the blood of patients.NS1 has been implicated in the pathogenesis of vascular permeability, but the mechanism is not completely understood. Using a complementary array of in vitroassays and disease relevant ECs and MCs, we described the possible roles for NS1 in dengue disease pathogenesis. Using microscopy and immunoblotting we observed that ECs internalize NS1 into endosomes, where it accumulates and is degraded overtime. Transcriptome and pathway analysis defined changes in global gene expression in ECs that are associated with cell dysfunction. We observed that NS1 induced an increase in multicellular rearrangements and a decrease in barrier function in ECs. We demonstrated that NS1-dependent activation of the p38 MAPK pathway controls the changes in EC permeability in vitro. Further, we discovered iiithat ECs and MCs respond to NS1 by secreting a specific array of proinflammatory cytokines and chemokines that may contribute to the cytokine storm in dengue disease. Finally, we found that NS1 internalization can mediate the uptake of bound antibodies into ECs. Together, these results suggest a vasoactive and proinflammatory role for DENV NS1 that may participate in the development of severe symptoms in dengue disease. The observed functions of NS1 could lead to the discovery of new therapeutic targets in dengue disease.

      Mitchell, David; Brandon, Smith (2013)
      Cilia and flagella are essential for the function of nearly all eukaryotes. This organelle is made up of nine outer doublet microtubules and two central singlet microtubules to form the canonical (9+2) ciliary structure. Cilia and flagella use this structure, as well as several protein complexes, such as the outer and inner dynein arms, the radial spokes, and the proteins that decorate the central pair to propagate the bending that produces motion. Flagellar motion is highly regulated, and each of these structures is necessary to regulate the dynein arms that generate the motile force. The central pair is one of the least understood of these structures. To date there are two major impediments hindering our understanding of the central pair: a lack of understanding as to how distinct central pair structures work in concert, and a general lack of available central pair mutant strains in the model organism Chlamydomonas reinhardtii. In order to further our understanding of how the central pair functions I have used multiple strategies. Firstly I have used previously characterized central pair mutants to study both structural interactions within the central pair and how the double mutant affects motility regulation. Secondly I provide evidence that a potential central pair mutant, H2, is indeed a central pair mutant and affects the C2b projection. Lastly I will attempt to characterize a new Chlamydomonas mutant, 10B5. Together these analyses will demonstrate that double mutants can have an additive effect on the structure of the central pair, and that double central pair mutants do not appear to suppress one another, but are at least ivepistatic to the most severe phenotype. I will also show evidence that 10B5 is not a central pair mutant, but with further study it may offer new insight into motilityregulation.
    • Expression and Function of Paxillin Genes in Zebrafish: A Role in Skeletal Muscle Development

      Turner, Chris; Amack, Jeffrey; Jacob, Andrew (2017)
      Paxillin is a key component of the Integrin adhesion complex, which regulates cellular signaling events in response to extracellular matrix interactions. Although the roles for Paxillin in cell migration have been extensively studied, less is understood about its role in vertebrate development. Depletion of Paxillin from mouse embryos results in early lethality due to impaired cardiovascular development and function, necessitating the development of alternative vertebrate genetic models for examining the role of Paxillin during embryogenesis. Zebrafish have emerged as an experimental vertebrate model amenable to genetic manipulation. The work compiled herein first characterizes the expression profiles for Paxillin genes in zebrafish, and then describes the embryonic phenotypes observed upon mutation of these genes. The identification of two Paxillin genes in zebrafish, pxnaand pxnb, provided new insight into the evolution of this gene family in the Teleost lineage. Both overlapping and unique expression profiles for these genes during zebrafish embryogenesis were uncovered. While both genes are expressed in developing skeletal muscle, pxnawas restricted to the notochord during earlier stages of embryogenesis and pxnbwas expressed in the developing heart. Targeted mutation of either gene alone did not impair embryonic development, suggesting partial functional redundancy between each gene during embryogenesis. Accordingly, combined mutations in pxnaand pxnbrevealed defects during the development ofseveral embryonic tissues. In particular, skeletal muscle morphogenesis iiiwas perturbed in these double mutant embryos. Further characterization revealed that Paxillin genes in zebrafish serve to regulate embryonic myotome shape and proper extracellular matrix composition during muscle development. The amount of Laminin was reduced, while the abundance of Fibronectin persisted, during myotome morphogenesis in Paxillin double mutant embryos. In addition, a role for cytoskeletal contractility in regulatingsubcellular localization of Paxillin in developing skeletal muscle was established. Defects in the development of the cardiovascular system were also apparent in Paxillin double mutant embryos, and future work will focus on characterizing these in further detail. Altogether, this work provides a new vertebrate model to use for understanding the role of Paxillin during embryonic development, and uncovers an unrecognized role for Paxillin in establishing the extracellular matrix of skeletal muscle.

      Moffat, Jennifer; DE, CHANDRAV (2015)
      The alphaherpesvirus varicella-zoster virus (VZV) is widespread in humans. VZV causes primary and recurrent diseases that are preventable with live vaccines and are treatable with antiviral drugs. New antiviral drugs for varicella-zoster virus (VZV) with increased potency are needed, especially to prevent post-herpetic neuralgia. The purpose of this project was to evaluate β-L-1-[5-(E-2-Bromovinyl)-2-(hydroxymethyl)-1,3-dioxolan-4- yl)] uracil (L-BHDU) and 5′-O-valyl-L-BHDU for efficacy, safety, resistance, and mechanism of action in three models of VZV replication: primary human foreskin fibroblasts (HFFs), skin organ culture (SOC) and in SCID-Hu mice with skin xenografts. We found that L-BHDU and valyl-L-BHDU were safe and effective against VZV in culture and in a mouse model. Herpes simplex virus Type 1 was also sensitive to LBHDU in cultured cells. The mechanism of action of L-BHDU and its effect on drugdrug interactions were not known. Given its similar structure to brivudine (BVdU), we addressed whether L-BHDU, like BVdU, inhibits 5-fluorouracil (5-FU) metabolism. LBHDU did not interfere with 5FU metabolism, indicating that L-BHDU is a safer drug than BVdU. However, L-BHDU antagonized the activity of acyclovir (ACV), BVdU and foscarnet (PFA) in cultured cells, which was due to competition for phosphorylation by VZV thymidine kinase (TK). The mechanism of action of L-BHDU was studied by evaluating its activity against related α-herpesviruses and by analyzing resistant VZV viii strains. VZV strains resistant to L-BHDU (L-BHDUR ) were cross-resistant to ACV and BVdU but not to PFA and cidofovir (CDV). Whole genome sequencing of L-BHDUR strains identified mutations in ATP-binding (G22R) and nucleoside binding (R130Q) domains of VZV TK. The purified L-BHDUR TKs were enzymatically inactive and failed to phosphorylate the drug. In wild type VZV- infected cells, L-BHDU was converted to L-BHDU mono- and diphosphate forms; cells infected with L-BHDUR virus did not phosphorylate the drug. We also investigated whether addition of nucleosides reversed LBHDU inhibition of VZV in dividing and quiescent HFFs. Excess thymidine and uridine, but not purines, in proportion to L-BHDU restored VZV replication only in dividing cells, suggesting that the active form of L-BHDU interfered with pyrimidine biosynthesis. Like other herpesviruses, VZV infection induced thymidine triphosphate (dTTP) in confluent cells while L-BHDU treatment decreased the dTTP pool. Some herpesviruses raise dNTP pools by inducing cellular enzymes. However, VZV infection did not increase cellular thymidylate synthase (TS) expression to facilitate viral replication. Furthermore, the active form of L-BHDU did not interfere with cellular metabolism, suggesting a viral target. Further studies are required to identify the target(s) of L-BHDU active form(s).
    • Biological importance of TIMP-2 phosphorylation on MMP-2 activity

      Bourboulia, Dimitra; Bullard, Renee (2016)
      Matrix metalloproteinases (MMPs) are proteolytic enzymes that are secreted from the cell and play an important role in embryonic development and tissue remodeling. In cancer, MMPs are hyperactive, promoting degradation of the ex-tracellular matrix. Enhancement of MMP proteolytic activity allows tumor cells to migrate and invade surrounding tissues, increasing the chance of metastasis. Tissue inhibitor of metalloproteinases (TIMPs) are also known to act extracellu-larly, and are the endogenous inhibitors of MMPs. To inhibit the protease activi-ty of MMPs, the N-terminus of the TIMP protein binds to the catalytic domain of MMP at a ratio of 1:1. Studies from our lab have found that TIMP-2 is phosphor-ylated on three tyrosine residues, and this phosphorylation increases the inter-action with MMP-2. This is the first time that phosphorylation of TIMP-2 has been reported. Fascinatingly, the proto-oncogene tyrosine kinase c-Src was found to phosphorylate TIMP-2. This is significant in that c-Src has not yet been shown to act extracellularly, and there are no details within the current lit-erature describing how this protein may function outside of the cell. In this the-sis, we usedmammalian cells as a model to decipher whether TIMP-2 phosphor-ylation wasable to occur extracellularly,as well as the effect that phosphoryla-tion of TIMP-2 hadon its functionto both inhibit/activate MMP-2. We found that(1) c-Src is able to phosphorylate TIMP-2 extracellularly in conditioned me-vidia; and (2) phosphorylation of TIMP-2 enhances its function of inhibiting MMP-2 proteolytic activity, as well as assisting in the activation of pro-MMP-2. Our results suggest the presence of anovel mechanismin whichphosphoryla-tion of TIMP-2is able to regulate the extracellular environment through en-hanced interaction with MMP-2. The information gained from this research couldlead to development of novel therapies that use phosphorylated TIMP-2 as a means of decreasing cellular migration and invasion with the overall goal of preventing metastasis.
    • “Children in Misery” or young crusaders?: the political utilization of children by the Women’s Christian Temperance Union

      Murphy, Shayna (2020-05)
      This paper discusses the Women’s Christian Temperance Union’s use of children for political purposes during their fight for Prohibition. In an effort to sympathize their mission and to create a sense of urgency around the banning of alcohol, members of the WCTU created an image of children as victims in their propaganda. However, the WCTU understood the importance of creating future voters, and so often created propaganda that presented children as active heroes. This conflicting portrayal of children showed that the WCTU used children as political tools and used such contrasting portrayals to reach a political goal rather than aptly represent children of alcoholic families. To understand this relationship between the WCTU and children, I analyzed posters created by the WCTU that present children as victims of alcohol and then content produced directly for children by the WCTU.
    • Chatbots: history, technology, and a case analysis

      Jay, Benjamin (2020-08)
      This thesis examines the more than 50 year history of chatbots that led to the development of Amazon’s Alexa, Google’s Assistant, and Apple’s Siri. A chatbot, commonly known as a conversational agent, is a computer framework that can have a normal conversation with a user by using a natural language processor (Reshmi and Balakrishnan, 2018). The goal is to understand the psychological and mathematical theories that worked well throughout history, as well as those that did not, and the impact they had on the evolution of modern chatbots. This thesis incorporates these theories into a new chatbot created using Google’s chatbot AI platform called Dialogflow. By following a Coursera course titled Building Conversational Experiences with Dialogflow, this thesis creates a chatbot that can schedule tours of a school and can answer questions about the SUNY New Paltz 2020 Commencement ceremony. Creating even the most basic chatbot requires a comprehensive understanding of the underlying theories and extensive coding experience (Abdul-Kader & Woods, 2015). This thesis assumes a foundation knowledge of computer coding.
    • The devil can cite scripture for his purpose: Shakespeare’s use of the parable of the Prodigal Son in ​Henry IV, The Merchant of Venice, King Lear​, and ​The Tempest

      Almeyda, Dariana (2020-05)
      Scholars have long identified the Bible as one of William Shakespeare’s main sources of inspiration. An extension to “The Devil Can Cite Scripture for His Purpose: Shakespeare’s Use of Biblical Allusions in ​The Merchant of Venice,”​ this paper explores Shakespeare’s implementation and reimagining of the parable of the Prodigal Son in ​Henry IV, The Merchant of Venice, King Lear,​ and ​The Tempest.​ His manipulation of the parable creates a universal sense of morality for the characters in each play and serves as a common ground for audiences of his time to understand and better relate to his works. To modern readers, his reworkings of the parable also serve as a social commentary on sixteenth-century English society steeped in religious conflicts and motifs. He creates several characters that act like prodigals, a term socially recognized by its relation to the parable found in Luke 15, but also universally understood as both an adjective and noun to mean “spending money or resources freely and recklessly; wastefully extravagant. / A person who spends money in a recklessly extravagant way” (“Prodigal”). ​Shakespeare’s various reworkings of this parable prompt a conversation about the price of forgiveness, love, and whether or not grace and mercy are truly free.
    • Exploring love languages: the key to building and maintaining healthy relationships

      Adams, Aryiah (2020-05)
      Communication is the heart of who we are as human beings. It is just as necessary as food and shelter because communication allows us to develop a civilized society that can transmit valuable information and knowledge. The desire to be loved and nurtured is also a fundamental human need that can be expressed through language. Through a series of interviews, the paper explores five “love languages” developed by Dr Gary Chapman used to communicate emotional fulfillment. The paper challenges the idea that time is a key component to the development of the five love languages. The research demonstrates that over time individuals discover their love language and that of their partner. Time further serves as a learning period that allows couples to recognize the emotional desires of their partner. Time then becomes the impetus for consistent acts of love creating growth between couples as they express love their partner accepts. The five love languages speak to the basic fundamentals needed to communicate love.
    • Designing an Instructional Informative Website for Pet Owners

      Jofre, Ana; Ortiz, Sherman; Jofre, Ana; First Reader; Lizardi, Ryan; Second Reader (SUNY Polytechnic Institute, 2020-12)
      Searching for pet owners' information on the world wide web can be difficult and confusing due to the many different websites on must scroll through depending on your search results. With the rise of social media, forums, online pet stores, and blogs there are more websites to look into. Although these websites have articles on pet information, they are often opinionated, lacking in follow up information or simply unfinished. This paper looks into the development of a site with the end users' navigation in mind for all their pets primary concerns. This website is made for user's accessing information about their pets. Using a classic template website builder, the site is able to be updated in real time with ease as more information becomes available. The website's user experience is evaluated by analyzing survey information. The end user experience, the most important aspect of any website, comprises navigation, clarity, and simplicity. Prototype website link:
    • Style Guide Development For An Internal Reference Library

      Lizardi, Ryan; Bush, Sarah C.; Lizardi, Ryan; First Reader; Jofre, Ana; Second Reader (SUNY Polytechnic Institute, 2020-12)
      This work strives to present information design and user experience (UX) techniques to improve continuity of design and usability, before migrating a cross-functional internal reference library to a new content management system (CMS). Expert-curated content accessed through LinkedIn Learning, combined with scholarly research, result in a complimentary style guide for use in the workplace.
    • Game Streaming in the Wake of a Pandemic: Topic: Live Streaming and Branding

      Jofre, Ana; Martucci, Nicholas; Jofre, Ana; First Reader; Lizardi, Ryan; Second Reader (SUNY Polytechnic Institute, 2020-12)
      The purpose of this study is to determine what the key motivational factors for creating a live stream gaming channel in the wake of a global pandemic are. This is executed by generating a series of podcast interviews from live streamers, generating branding for the launch of a live stream gaming channel and launching the channel. With the world in a current state of emergency, the live streaming industry under has boomed, giving streamers and viewers alike an opportunity to interact, communicate, and form communities like never before in the shadow of these pressing times. In light of this, we seek an escape from the harsh reality that is quarantine and find comfort in engaging with others all the while having fun indoors.
    • Critical Document Design: A Survey of Considerations for the Next Generation of Procedures Used in High-Risk Organizations

      Jofre, Ana; McLaren, Elizabeth A.; Jofre, Ana; First Reader; Lizardi, Ryan; Second Reader (SUNY Polytechnic Institute, 2020-12)
      Since the innovation of the pre-flight cockpit checklist first made the Boeing B-17 safe to fly, several high-risk industries have adopted the approach, to varying degrees. This paper reviews regulatory findings and recommendations from formal investigations following incidents across multiple high-risk industries to identify areas how checklists may be misused, misunderstood, or where they could have been of value to operators who didn't use them. The recommendations are then compared against current findings in information design and usability, and conclude with recommendations for how the future of electronic documentation may further improve the usability experience of operators and their teams, and ensure the safety of the public around them.