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dc.contributor.authorRoblin, P M
dc.contributor.authorHammerschlag, M R
dc.contributor.authorCummings, C
dc.contributor.authorWilliams, T H
dc.contributor.authorWorku, M
dc.date.accessioned2023-06-07T15:36:58Z
dc.date.available2023-06-07T15:36:58Z
dc.date.issued1989-05
dc.identifier.citationRoblin PM, Hammerschlag MR, Cummings C, Williams TH, Worku M. Comparison of two rapid microscopic methods and culture for detection of Chlamydia trachomatis in ocular and nasopharyngeal specimens from infants. J Clin Microbiol. 1989 May;27(5):968-70. doi: 10.1128/jcm.27.5.968-970.1989. PMID: 2663920; PMCID: PMC267464.en_US
dc.identifier.issn0095-1137
dc.identifier.pmid2663920
dc.identifier.urihttp://hdl.handle.net/20.500.12648/9907
dc.description.abstractThe data available for the diagnosis of chlamydial infections in infants which compare direct fluorescent-monoclonal-antibody stains (DFAs) with culture are limited to one reagent, MicroTrak (Syva Inc., Palo Alto, Calif.). We therefore performed a comparison of Pathfinder (Kallestad Diagnostics, Chaska, Minn.) and MicroTrak with chlamydia culture. Paired conjunctival and nasopharyngeal specimens for DFAs and cultures were obtained from 56 infants less than 1 month of age with conjunctivitis. The sensitivities for detecting C. trachomatis in conjunctival specimens with MicroTrak and Pathfinder were 93.8 and 88.2%, respectively, and the specificities were 87.5 and 94.9%, respectively. The DFA tests on nasopharyngeal specimens from infants with conjunctivitis did not perform as well. The sensitivities for Pathfinder and MicroTrak were 33 and 50%, respectively. There were a total of six patients with culture-positive chlamydial conjunctivitis whose nasopharyngeal specimens were DFA positive and culture negative; four of the specimens were positive by both DFAs. These six discordant specimens were further evaluated by preparing pellets and smears of the original culture specimens. All six contained typical fluorescing elementary bodies when stained with the Syva DFA reagent.
dc.language.isoenen_US
dc.relation.urlhttps://journals.asm.org/doi/10.1128/jcm.27.5.968-970.1989en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleComparison of two rapid microscopic methods and culture for detection of Chlamydia trachomatis in ocular and nasopharyngeal specimens from infants.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleJournal of clinical microbiologyen_US
dc.source.volume27
dc.source.issue5
dc.source.beginpage968
dc.source.endpage70
dc.source.countryUnited States
dc.description.versionVoRen_US
refterms.dateFOA2023-06-07T15:37:00Z
html.description.abstractThe data available for the diagnosis of chlamydial infections in infants which compare direct fluorescent-monoclonal-antibody stains (DFAs) with culture are limited to one reagent, MicroTrak (Syva Inc., Palo Alto, Calif.). We therefore performed a comparison of Pathfinder (Kallestad Diagnostics, Chaska, Minn.) and MicroTrak with chlamydia culture. Paired conjunctival and nasopharyngeal specimens for DFAs and cultures were obtained from 56 infants less than 1 month of age with conjunctivitis. The sensitivities for detecting C. trachomatis in conjunctival specimens with MicroTrak and Pathfinder were 93.8 and 88.2%, respectively, and the specificities were 87.5 and 94.9%, respectively. The DFA tests on nasopharyngeal specimens from infants with conjunctivitis did not perform as well. The sensitivities for Pathfinder and MicroTrak were 33 and 50%, respectively. There were a total of six patients with culture-positive chlamydial conjunctivitis whose nasopharyngeal specimens were DFA positive and culture negative; four of the specimens were positive by both DFAs. These six discordant specimens were further evaluated by preparing pellets and smears of the original culture specimens. All six contained typical fluorescing elementary bodies when stained with the Syva DFA reagent.
dc.description.institutionSUNY Downstateen_US
dc.description.departmentPediatricsen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalJournal of clinical microbiology


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Attribution-NonCommercial-NoDerivatives 4.0 International
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