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dc.contributor.authorGao, Peng P
dc.contributor.authorGraham, Joseph W
dc.contributor.authorZhou, Wen-Liang
dc.contributor.authorJang, Jinyoung
dc.contributor.authorAngulo, Sergio
dc.contributor.authorDura-Bernal, Salvador
dc.contributor.authorHines, Michael
dc.contributor.authorLytton, William W
dc.contributor.authorAntic, Srdjan D
dc.date.accessioned2023-04-10T15:57:36Z
dc.date.available2023-04-10T15:57:36Z
dc.date.issued2020-10-21
dc.identifier.citationGao PP, Graham JW, Zhou WL, Jang J, Angulo S, Dura-Bernal S, Hines M, Lytton WW, Antic SD. Local glutamate-mediated dendritic plateau potentials change the state of the cortical pyramidal neuron. J Neurophysiol. 2021 Jan 1;125(1):23-42. doi: 10.1152/jn.00734.2019. Epub 2020 Oct 21. PMID: 33085562; PMCID: PMC8087381.en_US
dc.identifier.eissn1522-1598
dc.identifier.doi10.1152/jn.00734.2019
dc.identifier.pmid33085562
dc.identifier.urihttp://hdl.handle.net/20.500.12648/8564
dc.description.abstractDendritic spikes in thin dendritic branches (basal and oblique dendrites) are traditionally inferred from spikelets measured in the cell body. Here, we used laser-spot voltage-sensitive dye imaging in cortical pyramidal neurons (rat brain slices) to investigate the voltage waveforms of dendritic potentials occurring in response to spatially restricted glutamatergic inputs. Local dendritic potentials lasted 200-500 ms and propagated to the cell body, where they caused sustained 10- to 20-mV depolarizations. Plateau potentials propagating from dendrite to soma and action potentials propagating from soma to dendrite created complex voltage waveforms in the middle of the thin basal dendrite, comprised of local sodium spikelets, local plateau potentials, and backpropagating action potentials, superimposed on each other. Our model replicated these voltage waveforms across a gradient of glutamatergic stimulation intensities. The model then predicted that somatic input resistance () and membrane time constant (tau) may be reduced during dendritic plateau potential. We then tested these model predictions in real neurons and found that the model correctly predicted the direction of and tau change but not the magnitude. In summary, dendritic plateau potentials occurring in basal and oblique branches put pyramidal neurons into an activated neuronal state ("prepared state"), characterized by depolarized membrane potential and smaller but faster membrane responses. The prepared state provides a time window of 200-500 ms, during which cortical neurons are particularly excitable and capable of following afferent inputs. At the network level, this predicts that sets of cells with simultaneous plateaus would provide cellular substrate for the formation of functional neuronal ensembles. In cortical pyramidal neurons, we recorded glutamate-mediated dendritic plateau potentials with voltage imaging and created a computer model that recreated experimental measures from dendrite and cell body. Our model made new predictions, which were then tested in experiments. Plateau potentials profoundly change neuronal state: a plateau potential triggered in one basal dendrite depolarizes the soma and shortens membrane time constant, making the cell more susceptible to firing triggered by other afferent inputs.
dc.language.isoenen_US
dc.relation.urlhttps://journals.physiology.org/doi/full/10.1152/jn.00734.2019en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectNMDA spikeen_US
dc.subjectensemblesen_US
dc.subjectintegrationen_US
dc.subjectmembrane time constanten_US
dc.subjectnonlinearen_US
dc.titleLocal glutamate-mediated dendritic plateau potentials change the state of the cortical pyramidal neuron.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleJournal of neurophysiologyen_US
dc.source.volume125
dc.source.issue1
dc.source.beginpage23
dc.source.endpage42
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.description.versionVoRen_US
refterms.dateFOA2023-04-10T15:57:37Z
html.description.abstractDendritic spikes in thin dendritic branches (basal and oblique dendrites) are traditionally inferred from spikelets measured in the cell body. Here, we used laser-spot voltage-sensitive dye imaging in cortical pyramidal neurons (rat brain slices) to investigate the voltage waveforms of dendritic potentials occurring in response to spatially restricted glutamatergic inputs. Local dendritic potentials lasted 200-500 ms and propagated to the cell body, where they caused sustained 10- to 20-mV depolarizations. Plateau potentials propagating from dendrite to soma and action potentials propagating from soma to dendrite created complex voltage waveforms in the middle of the thin basal dendrite, comprised of local sodium spikelets, local plateau potentials, and backpropagating action potentials, superimposed on each other. Our model replicated these voltage waveforms across a gradient of glutamatergic stimulation intensities. The model then predicted that somatic input resistance () and membrane time constant (tau) may be reduced during dendritic plateau potential. We then tested these model predictions in real neurons and found that the model correctly predicted the direction of and tau change but not the magnitude. In summary, dendritic plateau potentials occurring in basal and oblique branches put pyramidal neurons into an activated neuronal state ("prepared state"), characterized by depolarized membrane potential and smaller but faster membrane responses. The prepared state provides a time window of 200-500 ms, during which cortical neurons are particularly excitable and capable of following afferent inputs. At the network level, this predicts that sets of cells with simultaneous plateaus would provide cellular substrate for the formation of functional neuronal ensembles. In cortical pyramidal neurons, we recorded glutamate-mediated dendritic plateau potentials with voltage imaging and created a computer model that recreated experimental measures from dendrite and cell body. Our model made new predictions, which were then tested in experiments. Plateau potentials profoundly change neuronal state: a plateau potential triggered in one basal dendrite depolarizes the soma and shortens membrane time constant, making the cell more susceptible to firing triggered by other afferent inputs.
dc.description.institutionSUNY Downstateen_US
dc.description.departmentPhysiology and Pharmacologyen_US
dc.description.departmentNathan Kline Institute for Psychiatric Researchen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalJournal of neurophysiology


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