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dc.contributor.authorZhu, Kevin J
dc.contributor.authorSuttner, Brittany
dc.contributor.authorKnee, Jackie
dc.contributor.authorCapone, Drew
dc.contributor.authorMoe, Christine L
dc.contributor.authorStauber, Christine E
dc.contributor.authorKonstantinidis, Kostas T
dc.contributor.authorWallach, Thomas E
dc.contributor.authorPickering, Amy J
dc.contributor.authorBrown, Joe
dc.date.accessioned2022-10-17T16:21:10Z
dc.date.available2022-10-17T16:21:10Z
dc.date.issued2022-05-18
dc.identifier.citationZhu KJ, Suttner B, Knee J, Capone D, Moe CL, Stauber CE, Konstantinidis KT, Wallach TE, Pickering AJ, Brown J. Elevated Fecal Mitochondrial DNA from Symptomatic Norovirus Infections Suggests Potential Health Relevance of Human Mitochondrial DNA in Fecal Source Tracking. Environ Sci Technol Lett. 2022 Jun 14;9(6):543-550. doi: 10.1021/acs.estlett.2c00140. Epub 2022 May 18. PMID: 35719858; PMCID: PMC9202355.en_US
dc.identifier.issn2328-8930
dc.identifier.doi10.1021/acs.estlett.2c00140
dc.identifier.pmid35719858
dc.identifier.urihttp://hdl.handle.net/20.500.12648/7758
dc.description.abstractAn end goal of fecal source tracking (FST) is to provide information on risk of transmission of waterborne illnesses associated with fecal contamination. Ideally, concentrations of FST markers in ambient waters would reflect exposure risk. Human mtDNA is an FST marker that is exclusively human in origin and may be elevated in feces of individuals experiencing gastrointestinal inflammation. In this study, we examined whether human mtDNA is elevated in fecal samples from individuals with symptomatic norovirus infections using samples from the United States (US), Mozambique, and Bangladesh. We quantified hCYTB484 (human mtDNA) and HF183/BacR287 (human-associated ) FST markers using droplet digital polymerase chain reaction. We observed the greatest difference in concentrations of hCYTB484 when comparing samples from individuals with symptomatic norovirus infections versus individuals without norovirus infections or diarrhea symptoms: log increase of 1.42 in US samples (3,820% increase, -value = 0.062), 0.49 in Mozambique (308% increase, -value = 0.061), and 0.86 in Bangladesh (648% increase, -value = 0.035). We did not observe any trends in concentrations of HF183/BacR287 in the same samples. These results suggest concentrations of fecal mtDNA may increase during symptomatic norovirus infection and that mtDNA in environmental samples may represent an unambiguously human source-tracking marker that correlates with enteric pathogen exposure risk.en_US
dc.language.isoenen_US
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acs.estlett.2c00140#en_US
dc.rights© 2022 The Authors. Published by American Chemical Society.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleElevated Fecal Mitochondrial DNA from Symptomatic Norovirus Infections Suggests Potential Health Relevance of Human Mitochondrial DNA in Fecal Source Tracking.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleEnvironmental science & technology lettersen_US
dc.source.volume9
dc.source.issue6
dc.source.beginpage543
dc.source.endpage550
dc.source.countryUnited States
dc.description.versionVoRen_US
refterms.dateFOA2022-10-17T16:21:10Z
dc.description.institutionSUNY Downstateen_US
dc.description.departmentPediatric Gastroenterologyen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalEnvironmental science & technology letters


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© 2022 The Authors. Published by American Chemical Society.
Except where otherwise noted, this item's license is described as © 2022 The Authors. Published by American Chemical Society.