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dc.contributor.authorNatarajan, Aswin
dc.contributor.authorNadarajah, Vidushan
dc.contributor.authorFelsovalyi, Klara
dc.contributor.authorWang, Wenjuan
dc.contributor.authorJeyachandran, Vivian R
dc.contributor.authorWasson, Riley A
dc.contributor.authorCardozo, Timothy
dc.contributor.authorBracken, Clay
dc.contributor.authorKrogsgaard, Michelle
dc.date.accessioned2022-09-27T19:05:37Z
dc.date.available2022-09-27T19:05:37Z
dc.date.issued2016-03-17
dc.identifier.citationNatarajan A, Nadarajah V, Felsovalyi K, Wang W, Jeyachandran VR, Wasson RA, Cardozo T, Bracken C, Krogsgaard M. Structural Model of the Extracellular Assembly of the TCR-CD3 Complex. Cell Rep. 2016 Mar 29;14(12):2833-45. doi: 10.1016/j.celrep.2016.02.081. Epub 2016 Mar 17. PMID: 26997265; PMCID: PMC4902171.en_US
dc.identifier.eissn2211-1247
dc.identifier.doi10.1016/j.celrep.2016.02.081
dc.identifier.pmid26997265
dc.identifier.urihttp://hdl.handle.net/20.500.12648/7588
dc.description.abstractAntigen recognition of peptide-major histocompatibility complexes (pMHCs) by T cells, a key step in initiating adaptive immune responses, is performed by the T cell receptor (TCR) bound to CD3 heterodimers. However, the biophysical basis of the transmission of TCR-CD3 extracellular interaction into a productive intracellular signaling sequence remains incomplete. Here we used nuclear magnetic resonance (NMR) spectroscopy combined with mutational analysis and computational docking to derive a structural model of the extracellular TCR-CD3 assembly. In the inactivated state, CD3γε interacts with the helix 3 and helix 4-F strand regions of the TCR Cβ subunit, whereas CD3δε interacts with the F and C strand regions of the TCR Cα subunit in this model, placing the CD3 subunits on opposing sides of the TCR. This work identifies the molecular contacts between the TCR and CD3 subunits, identifying a physical basis for transmitting an activating signal through the complex.en_US
dc.language.isoenen_US
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S2211124716302169en_US
dc.rightsCopyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleStructural Model of the Extracellular Assembly of the TCR-CD3 Complex.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleCell reportsen_US
dc.source.volume14
dc.source.issue12
dc.source.beginpage2833
dc.source.endpage45
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.description.versionVoRen_US
refterms.dateFOA2022-09-27T19:05:38Z
dc.description.institutionSUNY Downstateen_US
dc.description.departmentOrthopaedic Surgery & Rehabilitation Medicineen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalCell reports


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Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
Except where otherwise noted, this item's license is described as Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.