Structural Model of the Extracellular Assembly of the TCR-CD3 Complex.
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Jeyachandran, Vivian R
Wasson, Riley A
Journal titleCell reports
Publication Begin page2833
Publication End page45
MetadataShow full item record
AbstractAntigen recognition of peptide-major histocompatibility complexes (pMHCs) by T cells, a key step in initiating adaptive immune responses, is performed by the T cell receptor (TCR) bound to CD3 heterodimers. However, the biophysical basis of the transmission of TCR-CD3 extracellular interaction into a productive intracellular signaling sequence remains incomplete. Here we used nuclear magnetic resonance (NMR) spectroscopy combined with mutational analysis and computational docking to derive a structural model of the extracellular TCR-CD3 assembly. In the inactivated state, CD3γε interacts with the helix 3 and helix 4-F strand regions of the TCR Cβ subunit, whereas CD3δε interacts with the F and C strand regions of the TCR Cα subunit in this model, placing the CD3 subunits on opposing sides of the TCR. This work identifies the molecular contacts between the TCR and CD3 subunits, identifying a physical basis for transmitting an activating signal through the complex.
CitationNatarajan A, Nadarajah V, Felsovalyi K, Wang W, Jeyachandran VR, Wasson RA, Cardozo T, Bracken C, Krogsgaard M. Structural Model of the Extracellular Assembly of the TCR-CD3 Complex. Cell Rep. 2016 Mar 29;14(12):2833-45. doi: 10.1016/j.celrep.2016.02.081. Epub 2016 Mar 17. PMID: 26997265; PMCID: PMC4902171.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
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