Show simple item record

dc.contributor.authorKolokotronis, Sergios-Orestis
dc.contributor.authorMacPhee, Ross D E
dc.contributor.authorGreenwood, Alex D
dc.date.accessioned2022-09-12T16:59:55Z
dc.date.available2022-09-12T16:59:55Z
dc.date.issued2007-04-27
dc.identifier.citationKolokotronis SO, Macphee RD, Greenwood AD. Detection of mitochondrial insertions in the nucleus (NuMts) of Pleistocene and modern muskoxen. BMC Evol Biol. 2007 Apr 27;7:67. doi: 10.1186/1471-2148-7-67. PMID: 17466066; PMCID: PMC1876215.en_US
dc.identifier.eissn1471-2148
dc.identifier.doi10.1186/1471-2148-7-67
dc.identifier.pmid17466066
dc.identifier.urihttp://hdl.handle.net/20.500.12648/7555
dc.description.abstractBackground: Nuclear insertions of mitochondrial sequences (NuMts) have been identified in a wide variety of organisms. Trafficking of genetic material from the mitochondria to the nucleus has occurred frequently during mammalian evolution and can lead to the production of a large pool of sequences with varying degrees of homology to organellar mitochondrial DNA (mtDNA) sequences. This presents both opportunities and challenges for forensics, population genetics, evolutionary genetics, conservation biology and the study of DNA from ancient samples. Here we present a case in which difficulties in ascertaining the organellar mtDNA sequence from modern samples hindered their comparison to ancient DNA sequences. Results: We obtained mitochondrial hypervariable region (HVR) sequences from six ancient samples of tundra muskox (Ovibos moschatus) that were reproducible but distinct from modern muskox sequences reported previously. Using the same PCR primers applied to the ancient specimens and the primers used to generate the modern muskox DNA sequences in a previous study, we failed to definitively identify the organellar sequence from the two modern muskox samples tested. Instead of anticipated sequence homogeneity, we obtained multiple unique sequences from both hair and blood of one modern specimen. Sequencing individual clones of a >1 kb PCR fragment from modern samples did not alleviate the problem as there was not a consistent match across the entire length of the sequences to Ovibos when compared to sequences in GenBank. Conclusion: In specific taxa, due to nuclear insertions some regions of the mitochondrial genome may not be useful for the characterization of modern or ancient DNA.en_US
dc.language.isoenen_US
dc.relation.urlhttps://bmcecolevol.biomedcentral.com/articles/10.1186/1471-2148-7-67en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDetection of mitochondrial insertions in the nucleus (NuMts) of Pleistocene and modern muskoxen.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleBMC evolutionary biologyen_US
dc.source.volume7
dc.source.beginpage67
dc.source.endpage
dc.source.countryEngland
dc.description.versionVoRen_US
refterms.dateFOA2022-09-12T16:59:55Z
dc.description.institutionSUNY Downstateen_US
dc.description.departmentEpidemiology and Biostatisticsen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalBMC evolutionary biology


Files in this item

Thumbnail
Name:
1471-2148-7-67.pdf
Size:
325.1Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International