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dc.contributor.authorLowenstein, Jacob H.
dc.contributor.authorAmato, George
dc.contributor.authorKolokotronis, Sergios-Orestis
dc.date.accessioned2022-09-12T16:10:39Z
dc.date.available2022-09-12T16:10:39Z
dc.date.issued2009-11-18
dc.identifier.citationLowenstein JH, Amato G, Kolokotronis SO. The real maccoyii: identifying tuna sushi with DNA barcodes--contrasting characteristic attributes and genetic distances. PLoS One. 2009 Nov 18;4(11):e7866. doi: 10.1371/journal.pone.0007866. PMID: 19924239; PMCID: PMC2773415.en_US
dc.identifier.eissn1932-6203
dc.identifier.doi10.1371/journal.pone.0007866
dc.identifier.pmid19924239
dc.identifier.urihttp://hdl.handle.net/20.500.12648/7547
dc.description.abstractBackground: The use of DNA barcodes for the identification of described species is one of the least controversial and most promising applications of barcoding. There is no consensus, however, as to what constitutes an appropriate identification standard and most barcoding efforts simply attempt to pair a query sequence with reference sequences and deem identification successful if it falls within the bounds of some pre-established cutoffs using genetic distance. Since the Renaissance, however, most biological classification schemes have relied on the use of diagnostic characters to identify and place species. Methodology/principal findings: Here we developed a cytochrome c oxidase subunit I character-based key for the identification of all tuna species of the genus Thunnus, and compared its performance with distance-based measures for identification of 68 samples of tuna sushi purchased from 31 restaurants in Manhattan (New York City) and Denver, Colorado. Both the character-based key and GenBank BLAST successfully identified 100% of the tuna samples, while the Barcode of Life Database (BOLD) as well as genetic distance thresholds, and neighbor-joining phylogenetic tree building performed poorly in terms of species identification. A piece of tuna sushi has the potential to be an endangered species, a fraud, or a health hazard. All three of these cases were uncovered in this study. Nineteen restaurant establishments were unable to clarify or misrepresented what species they sold. Five out of nine samples sold as a variant of "white tuna" were not albacore (T. alalunga), but escolar (Lepidocybium flavorunneum), a gempylid species banned for sale in Italy and Japan due to health concerns. Nineteen samples were northern bluefin tuna (T. thynnus) or the critically endangered southern bluefin tuna (T. maccoyii), though nine restaurants that sold these species did not state these species on their menus. Conclusions/significance: The Convention on International Trade Endangered Species (CITES) requires that listed species must be identifiable in trade. This research fulfills this requirement for tuna, and supports the nomination of northern bluefin tuna for CITES listing in 2010.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)en_US
dc.relation.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0007866en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMultidisciplinaryen_US
dc.titleThe Real maccoyii: Identifying Tuna Sushi with DNA Barcodes – Contrasting Characteristic Attributes and Genetic Distancesen_US
dc.typeArticle/Reviewen_US
dc.source.journaltitlePLoS ONEen_US
dc.source.volume4
dc.source.issue11
dc.source.beginpagee7866
dc.description.versionVoRen_US
refterms.dateFOA2022-09-12T16:10:40Z
dc.description.institutionSUNY Downstateen_US
dc.description.departmentEpidemiology and Biostatisticsen_US
dc.description.degreelevelN/Aen_US


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