• Login
    View Item 
    •   Home
    • Doctoral Degree Granting Institutions
    • SUNY Polytechnic Institute
    • SUNY Polytechnic Institute Doctoral Dissertations
    • Colleges of Nanoscale Science and Engineering Doctoral Dissertations
    • View Item
    •   Home
    • Doctoral Degree Granting Institutions
    • SUNY Polytechnic Institute
    • SUNY Polytechnic Institute Doctoral Dissertations
    • Colleges of Nanoscale Science and Engineering Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SUNY Open Access RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsDepartmentThis CollectionPublication DateAuthorsTitlesSubjectsDepartmentAuthor ProfilesView

    My Account

    LoginRegister

    Campus Communities in SOAR

    Alfred State CollegeBrockportBroomeCantonDownstateEmpireFashion Institute of TechnologyFredoniaMaritimeNew PaltzOneontaOptometryOswegoPlattsburghSUNY Polytechnic InstituteSUNY PressUpstate Medical

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Microfluidic Imaging Windows for Study of the Tumor Microenvironment

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    TristanHeadDissertation-final- ...
    Size:
    8.013Mb
    Format:
    PDF
    Description:
    Final Dissertation Submission
    Download
    Thumbnail
    Name:
    THeadDistributionLicense2022.pdf
    Size:
    584.7Kb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Author
    Head, Tristen
    Keyword
    Tumor microenvironment (TME)
    Microfabrication techniques
    Microfluidic imaging window (MFIW)
    Micro-nozzles
    Readers/Advisors
    Tokranova, Natalya
    Xie, Yubing
    Boivin, Benoit
    Entenberg, David
    Cady, Nathaniel C., Dissertation Committee Chair
    Date Published
    2022-08
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.12648/7533
    Abstract
    Despite decades of research and billions of dollars in funding, cancer has maintained its epidemiological prominence as the second leading cause of death in the US for nearly 90 years. Currently, the clinical trial success rates for oncologic drugs is ~3%, and approved drugs often have a modest impact on overall survival. This is due in part to the tumor microenvironment (TME) which promotes cancer development and mitigates therapeutic response. Study of this biological system, however, is limited by conventional in vitro and in vivo techniques, which compromise either physiological relevance or experimental control. To better understand the role of the TME, we have utilized microfabrication techniques to develop the microfluidic imaging window (MFIW), an implantable platform for the observation and manipulation of in vivo TMEs. This technology provides unique opportunities for assessing the pharmacologic effects of therapeutics within intact, living tissue. Among the applications explored, a novel photolithographic technique, termed post exposure lamination, was developed to integrate tapered SU-8 micro-nozzle structures and enhance fluid conduction into porous matrices. Using these features, it was found that micro-nozzles improved axial penetration of fluorescent dextran into agarose tissue mimics and reduced the radial dispersion of Trypan Blue dye. Applications of localized reagent delivery for enhanced assay control were also investigated using small molecule nuclear stains and cell-based reporter systems. Here, significant cell staining occurred rapidly using small volumes of reagent (100 nL), substrate delivery for enzymatic processing was detected using a bioluminescent readout, and induction of cell gene expression was used to upregulate the production of fluorescent protein. Collectively, these capabilities showcase applications of the MFIW for enhanced monitoring and modulation of the TME that are well suited for translation into in vivo animal studies.
    Collections
    Colleges of Nanoscale Science and Engineering Doctoral Dissertations

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.