Evolution of hypervirulence by a MRSA clone through acquisition of a transposable element
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorBenson, Meredith A.
Ohneck, Elizabeth A.
Satola, Sarah W.
Planet, Paul J.
Torres, Victor J.
Journal titleMolecular Microbiology
Publication Begin page664
Publication End page681
MetadataShow full item record
AbstractStaphylococcus aureus has evolved as a pathogen that causes a range of diseases in humans. There are two dominant modes of evolution thought to explain most of the virulence differences between strains. First, virulence genes may be acquired from other organisms. Second, mutations may cause changes in the regulation and expression of genes. Here we describe an evolutionary event in which transposition of an IS element has a direct impact on virulence gene regulation resulting in hypervirulence. Whole-genome analysis of a methicillin-resistant S. aureus (MRSA) strain USA500 revealed acquisition of a transposable element (IS256) that is absent from close relatives of this strain. Of the multiple copies of IS256 found in the USA500 genome, one was inserted in the promoter sequence of repressor of toxins (Rot), a master transcriptional regulator responsible for the expression of virulence factors in S. aureus. We show that insertion into the rot promoter by IS256 results in the derepression of cytotoxin expression and increased virulence. Taken together, this work provides new insight into evolutionary strategies by which S. aureus is able to modify its virulence properties and demonstrates a novel mechanism by which horizontal gene transfer directly impacts virulence through altering toxin regulation.
CitationBenson MA, Ohneck EA, Ryan C, Alonzo F 3rd, Smith H, Narechania A, Kolokotronis SO, Satola SW, Uhlemann AC, Sebra R, Deikus G, Shopsin B, Planet PJ, Torres VJ. Evolution of hypervirulence by a MRSA clone through acquisition of a transposable element. Mol Microbiol. 2014 Aug;93(4):664-81. doi: 10.1111/mmi.12682. Epub 2014 Jul 16. PMID: 24962815; PMCID: PMC4127135.
The following license files are associated with this item:
- Creative Commons
- CodY deletion enhances in vivo virulence of community-associated methicillin-resistant Staphylococcus aureus clone USA300.
- Authors: Montgomery CP, Boyle-Vavra S, Roux A, Ebine K, Sonenshein AL, Daum RS
- Issue date: 2012 Jul
- Decreased vancomycin susceptibility in Staphylococcus aureus caused by IS256 tempering of WalKR expression.
- Authors: McEvoy CR, Tsuji B, Gao W, Seemann T, Porter JL, Doig K, Ngo D, Howden BP, Stinear TP
- Issue date: 2013 Jul
- Origin and evolution of European community-acquired methicillin-resistant Staphylococcus aureus.
- Authors: Stegger M, Wirth T, Andersen PS, Skov RL, De Grassi A, Simões PM, Tristan A, Petersen A, Aziz M, Kiil K, Cirković I, Udo EE, del Campo R, Vuopio-Varkila J, Ahmad N, Tokajian S, Peters G, Schaumburg F, Olsson-Liljequist B, Givskov M, Driebe EE, Vigh HE, Shittu A, Ramdani-Bougessa N, Rasigade JP, Price LB, Vandenesch F, Larsen AR, Laurent F
- Issue date: 2014 Aug 26
- Daptomycin resistance in methicillin-resistant Staphylococcus aureus is conferred by IS256 insertion in the promoter of mprF along with mutations in mprF and walK.
- Authors: Yin Y, Chen H, Li S, Gao H, Sun S, Li H, Wang R, Jin L, Liu Y, Wang H
- Issue date: 2019 Dec
- Demography and Intercontinental Spread of the USA300 Community-Acquired Methicillin-Resistant Staphylococcus aureus Lineage.
- Authors: Glaser P, Martins-Simões P, Villain A, Barbier M, Tristan A, Bouchier C, Ma L, Bes M, Laurent F, Guillemot D, Wirth T, Vandenesch F
- Issue date: 2016 Feb 16