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dc.contributor.authorBotelho, Ana Maria Nunes
dc.contributor.authorCerqueira e Costa, Maiana Oliveira
dc.contributor.authorMoustafa, Ahmed M.
dc.contributor.authorBeltrame, Cristiana Ossaille
dc.contributor.authorFerreira, Fabienne Antunes
dc.contributor.authorCôrtes, Marina Farrel
dc.contributor.authorCosta, Bruno Souza Scramignon
dc.contributor.authorSilva, Deborah Nascimento Santos
dc.contributor.authorBandeira, Paula Terra
dc.contributor.authorLima, Nicholas Costa Barroso
dc.contributor.authorSouza, Rangel Celso
dc.contributor.authorAlmeida, Luiz Gonzaga Paula de
dc.contributor.authorVasconcelos, Ana Tereza Ribeiro
dc.contributor.authorNarechania, Apurva
dc.contributor.authorRyan, Chanelle
dc.contributor.authorO’Brien, Kelsey
dc.contributor.authorKolokotronis, Sergios-Orestis
dc.contributor.authorPlanet, Paul J.
dc.contributor.authorNicolás, Marisa Fabiana
dc.contributor.authorFigueiredo, Agnes Marie Sá
dc.description.abstractThe global spread of specific clones of methicillin-resistant Staphylococcus aureus (MRSA) has become a major public health problem, and understanding the dynamics of geographical spread requires worldwide surveillance. Over the past 20 years, the ST239 lineage of MRSA has been recognized as an emerging clone across the globe, with detailed studies focusing on isolates from Europe and Asia. Less is known about this lineage in South America, and, particularly, Brazil where it was the predominant lineage of MRSA in the early 1990s to 2000s. To gain a better understanding about the introduction and spread of ST239 MRSA in Brazil we undertook a comparative phylogenomic analysis of ST239 genomes, adding seven completed, closed Brazilian genomes. Brazilian ST239 isolates grouped in a subtree with those from South American, and Western, romance-language-speaking, European countries, here designated the South American clade. After an initial worldwide radiation in the 1960s and 1970s, we estimate that ST239 began to spread in South America and Brazil in approximately 1988. This clone demonstrates specific genomic changes that are suggestive of local divergence and adaptational change including agrC single-nucleotide polymorphisms variants, and a distinct pattern of virulence-associated genes (mainly the presence of the chp and the absence of sea and sasX). A survey of a geographically and chronologically diverse set of 100 Brazilian ST239 isolates identified this virulence genotype as the predominant pattern in Brazil, and uncovered an unexpectedly high prevalence of agr-dysfunction (30%). ST239 isolates from Brazil also appear to have undergone transposon (IS256) insertions in or near global regulatory genes (agr and mgr) that likely led to rapid reprogramming of bacterial traits. In general, the overall pattern observed in phylogenomic analyses of ST239 is of a rapid initial global radiation, with subsequent local spread and adaptation in multiple different geographic locations. Most ST239 isolates harbor the ardA gene, which we show here to have in vivo anti-restriction activity. We hypothesize that this gene may have improved the ability of this lineage to acquire multiple resistance genes and distinct virulence-associated genes in each local context. The allopatric divergence pattern of ST239 also may suggest strong selective pressures for specific traits in different geographical locations.en_US
dc.description.sponsorshipFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiroen_US
dc.publisherFrontiers Media SAen_US
dc.rightsAttribution 4.0 International*
dc.subjectMicrobiology (medical)en_US
dc.subjectBrazilian epidemic cloneen_US
dc.subjectBrazilian/Hungarian cloneen_US
dc.subjectcomparative genomicsen_US
dc.subjectmethicillin-resistant Staphylococcus aureusen_US
dc.titleLocal Diversification of Methicillin- Resistant Staphylococcus aureus ST239 in South America After Its Rapid Worldwide Disseminationen_US
dc.source.journaltitleFrontiers in Microbiologyen_US
dc.description.institutionSUNY Downstateen_US
dc.description.departmentEpidemiology and Biostatisticsen_US

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Attribution 4.0 International
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