Characterization of Harvard Forest bacterial isolates: drought and copper tolerance

Abstract

This research seeks to better understand if two whole genome sequenced isolates, BS40 and BS71, from a longitudinal climate change mimic study, possess genes that are indicative of drought resistance. This can help us make inferences on evolutionary adaptions in response to soil warming. This research describes the bioinformatic analysis of potentially acquired drought resistance genes within the two isolated strains BS40 and BS71. It also details the subsequent bioinformatic and experimental analysis of BS71’s copper resistance as it examines the potential copper resistance and detoxification abilities of BS71. It also explores the modulation of copper resistance by nutrient deficiency and BS71’s potential to be utilized in bioremediation.