Predicted Metabolic Function of the Gut Microbiota of Drosophila melanogaster
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Keyword
Computer Science ApplicationsGenetics
Molecular Biology
Modelling and Simulation
Ecology, Evolution, Behavior and Systematics
Biochemistry
Physiology
Microbiology
microbiome
constraint-based modeling
mutualism
competition
cross-feeding
Drosophila
Journal title
mSystemsDate Published
2021-06-29Publication Volume
6Publication Issue
3
Metadata
Show full item recordAbstract
An important goal for many nutrition-based microbiome studies is to identify the metabolic function of microbes in complex microbial communities and their impact on host physiology. This research can be confounded by poorly understood effects of community composition and host diet on the metabolic traits of individual taxa. Here, we investigated these multiway interactions by constructing and analyzing metabolic models comprising every combination of five bacterial members of the Drosophila gut microbiome (from single taxa to the five-member community of Acetobacter and Lactobacillus species) under three nutrient regimes. We show that the metabolic function of Drosophila gut bacteria is dynamic, influenced by community composition, and responsive to dietary modulation. Furthermore, we show that ecological interactions such as competition and mutualism identified from the growth patterns of gut bacteria are underlain by a diversity of metabolic interactions, and show that the bacteria tend to compete for amino acids and B vitamins more frequently than for carbon sources. Our results reveal that, in addition to fermentation products such as acetate, intermediates of the tricarboxylic acid (TCA) cycle, including 2-oxoglutarate and succinate, are produced at high flux and cross-fed between bacterial taxa, suggesting important roles for TCA cycle intermediates in modulating Drosophila gut microbe interactions and the potential to influence host traits. These metabolic models provide specific predictions of the patterns of ecological and metabolic interactions among gut bacteria under different nutrient regimes, with potentially important consequences for overall community metabolic function and nutritional interactions with the host.Citation
Ankrah, N. Y. D., Barker, B. E., Song, J., Wu, C., McMullen, J. G., & Douglas, A. E. (2021). Predicted metabolic function of the gut microbiota of Drosophila melanogaster. MSystems, 6(3). https://doi.org/10.1128/mSystems.01369-20DOI
10.1128/msystems.01369-20ae974a485f413a2113503eed53cd6c53
10.1128/msystems.01369-20
Scopus Count
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/