• Login
    View Item 
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Senior Honors Theses
    • View Item
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Senior Honors Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SUNY Open Access RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsDepartmentThis CollectionPublication DateAuthorsTitlesSubjectsDepartmentAuthor ProfilesView

    My Account

    LoginRegister

    Campus Communities in SOAR

    Alfred State CollegeBrockportBroomeCantonDownstateEmpireFashion Institute of TechnologyFredoniaMaritimeNew PaltzOneontaOptometryOswegoPlattsburghSUNY Polytechnic InstituteSUNY PressUpstate Medical

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Disruption of Caveolae Lipid Rafts and the Effects on Melanin-concentrating Hormone Receptor-1 Localization: A Pharmacological Study

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    honors/56/fulltext (1).pdf
    Size:
    512.0Kb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Author
    King, Colin
    Keyword
    Brockport Honors College
    Biology
    Pharmacology
    MCH
    Date Published
    2014-05-15
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.12648/6806
    Abstract
    Melanin-concentrating hormone (MCH) is integral to the regulation of human appetite. MCH targets G protein-coupled receptors in the brain and peripheral tissues. When MCH receptor 1 binds MCH on the surface of cells, it activates multiple signaling pathways, then desensitizes. Internalization of MCH-bound MCHR1 is only thought to be partially responsible for the loss of MCH signaling capacity of cells. Previous research has shown that MCH receptors are enriched in caveolae and specifically complex with caveolin-1. Caveolin-1 is a key structural component of caveolae, which are cholesterol-based lipid rafts that are known for concentrating signaling molecules and clathrin-independent endocytosis. This study aims to investigate how MCH signaling would be affected if MCH receptors weren’t enriched in these regions. Pharmacological treatments were used to achieve this goal as caveolae formation was disrupted with the antibiotic nystatin, a cholesterol inhibitor. It has been shown that sodium carbonate-based extraction procedure followed by flotation on sucrose density centrifugation isolates caveolae from other cell contents. Caveolae-isolation procedures to detect caveloin-1 were undertaken to indicate that untreated BHK-570 cells contained caveolin-1 in fractions 4 and 5 of the sucrose gradients while a gradient shift of caveolin-1 to fractions 7-10 in nystatin-treated cells occurred. Such shift confirmed that there was a partial disruption of caveolae within the treated cells. Future experiments will test whether other pharmacological inhibitors such as filipin and methyl-?-cyclodextrin as well as caveolin-1 RNAi are better able to deplete caveolae from cells as well as their impact on MCH signaling.
    Collections
    Senior Honors Theses

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.