An In Ovo Study of Actinomycin-D Effects on Early Chick Development
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Author
McCoshen, John AdrianDate Published
1971-08-01
Metadata
Show full item recordAbstract
The effects of actinomycin-D on stage 1 chick blastoderms has been studied in ovo. After 48 hours exposure to 2.0 ug actinomycin-D/egg, blood island formation took place while all other organogenesis was inhibited. Visual observation of treated blastoderms indicated hemoglobin synthesis to have taken place also. These results imply that a mRNA(s) is transcribed by stage 1 in chick for blood island development. Histosections imply that translation of this system takes place about 26 hours or less after stage 1 treatment. Preliminary tests with the fluorescent dye, acridine orange, specific for RNA and DNA, indicated that neo-synthesis of RNA was inhibited 6 hours after treatment. Some cells were found to contain abundant RNA in the cytoplasm which may be the precursor information for the blood vascular system. A new in ovo technique designed to overcome anomalous development due to handling was designed for this study. Precautions to in ovo culturing are described and a possible cause of anomalous in ovo development is discussed.