Assessing the Potential of PRTOLl as a Bioremedial Agent Using Non-Isotopic, 16S-rRNA Targeted Oligonucleotide Probes

Abstract

This investigation focuses on the bioremediation of toluene in freshwater, anaerobic conditions by the recently isolated bacterial strain PRTOL1, and the development of a rapid and efficient quantitative method using 16S-rRNA targeted, fluorescently labelled oligonucleotide probes to directly detect and monitor this strain in situ. This investigation also focuses on the application of this method to determine the effects of amending the soil with various carbon sources on the population of PRTOL1.