Modified Nucleotides of Mitochondrial Transfer Ribonucleic Acids

Abstract

32P-labeled low molecular weight RNA was extracted from mitochondria of KB cells. Thin layer chromatography was used to separate and quantitate the various nucleotides. The amount of label incorporated into modified nucleotides of mitochondrial LMW RNA was 5.6% of the total amount incorporated into all nucleotides of this RNA. The value obtained in the same way for cytoplasmic LMW RNA was 10.9%. This is the only data available on modified nucleotide content of mitochondrial LMW RNA.