• Login
    View Item 
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Biology Master’s Theses
    • View Item
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Biology Master’s Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SUNY Open Access RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsDepartmentThis CollectionPublication DateAuthorsTitlesSubjectsDepartmentAuthor ProfilesView

    My Account

    LoginRegister

    Campus Communities in SOAR

    Alfred State CollegeBrockportBroomeCantonDownstateEmpireFredoniaMaritimeNew PaltzOneontaOptometryOswegoPlattsburghSUNY Polytechnic InstituteSUNY Office of Community Colleges and the Education PipelineSUNY PressUpstate Medical

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Premature Chromosome Condensation of Synchronized Chinese Hamster Lung Cells

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    bio_theses/56/fulltext (1).pdf
    Size:
    2.888Mb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Author
    Tucci, Steven Michael
    Keyword
    Metaphase-S Fusions
    Mitotic Nuclei
    Allantoic Cavity
    Chromatin
    Sendai Virus
    Date Published
    1977-06-01
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.12648/4530
    Abstract
    The orderly, nonrandom pattern of nuclear chromatin is observable when it is condensed as visually distinct and structurally reproducible metaphase chromosomes. Interphase nuclei are susceptible to being induced to condense prematurely when by somatic cell fusion they become exposed to the influence of mitotic nuclei. The mitotic inducer has a differential effect on G_1, S, and G­_2 chromatin and produces varied premature condensation products. In the following experiments metaphase-G_1 fusions yielded single, elongated chromatids. The metaphase-S fusions resulted in pulverized condensation products. Metaphase-G_2 fusions produced sister, elongated chromatids. Sendai virus was used as the fusing agent in these experiments. The seed virus was injected into the allantoic cavity of 11-day chick embryos. Four days post injection the allantoic fluid was collected and titrated to give the desired virus concentration. The virus was subsequently inactivated by ultraviolet exposure. This inactive Sendai virus when suspended in medium with the Chinese hamster cells, produced cell fusion and the various premature condensation products. The percentages of these various fusion products were able to be influenced by using synchronized cell populations. Thymidine, hydroxyurea and colcemid were the drugs of choice to bring about synchrony. Both thymidine and hydroxyurea have the capability to produce a transient inhibition of DNA synthesis. Colcemid interferes with the polymerization of microtubules and therefore arrests cells in metaphase. When synchronized populations were fused and compared to asynchronous population fusions.
    Collections
    Biology Master’s Theses

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.