• Login
    View Item 
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Biology Master’s Theses
    • View Item
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Biology Master’s Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SUNY Open Access RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsDepartmentThis CollectionPublication DateAuthorsTitlesSubjectsDepartmentAuthor ProfilesView

    My Account

    LoginRegister

    Campus Communities in SOAR

    Alfred State CollegeBrockportBroomeCantonDownstateEmpireFredoniaMaritimeNew PaltzOneontaOptometryOswegoPlattsburghSUNY Polytechnic InstituteSUNY Office of Community Colleges and the Education PipelineSUNY PressUpstate Medical

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    The Role of Ilv5p Interacting Factors in Mitochondrial DNA Stability

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    bio_theses/5/fulltext (1).pdf
    Size:
    7.325Mb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Author
    Mirando, Anthony J.
    Keyword
    Brockport Biology Thesis
    Yeast Two-Hybrid Assay
    Mitochondrial DNA
    Gene Disruption
    Date Published
    2006-06-20
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.12648/4522
    Abstract
    The ease of manipulating yeast allows for advanced studies on the factors affecting the mitochondrial DNA mutation rates. The control mechanisms of the mitochondrial DNA mutation rate has been determined to involve the dual function protein, Ilv5p. The Ilv5p plays an integral role in the proper segregation of newly replicated mitochondrial DNA into daughter cells during cell division. The focus of this study is to find unknown factors involved in mitochondrial DNA stability. This study uses the Ilv5p to pull unknown factors out of the many genes that comprise the yeast genome. The identification of interacting factors of the Ilv5p is the focus of this study. We have found proteins likely to physically interact with the Ilv5p and play a role in the stabilization of mtDNA. These proteins have been identified as the Vma8p and the YOL057W gene product. These proteins have been tested and shown to affect the stability of mitochondrial DNA. The Vma8p is the D subunit that comprises the V 1 complex. The V-ATPase that is responsible for ATP synthesis possesses two complexes, the V_1 and the V_0 complex. The V_1 and the V_0 complexes readily dissociate and reassociate in response to environmental conditions optimizing acidification of vacuole components for regulation of ATPase activity in other cellular components. The loss of this particular protein increases the occurrence of ?-petite or non-functional mitochondria. A microsatellite instability assay showed that the mutation rate increased 50 times with the loss of the Vma8p. The respiration loss assay showed the loss of the YOL057W gene product caused a significant increase in the rate of ?- petite formations. This assay is the first to show that the putative open reading frame (ORF) encodes a protein whose loss of function leads to a mitochondrial defect. The exact role of this particular protein in the mitochondria has yet to be determined.
    Collections
    Biology Master’s Theses

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.