Show simple item record

dc.contributor.advisorMcLean, Robert J.
dc.contributor.authorColombino, Luciano Francis
dc.date.accessioned2021-09-07T21:02:41Z
dc.date.available2021-09-07T21:02:41Z
dc.date.issued1/1/1976
dc.identifier.urihttp://hdl.handle.net/20.500.12648/4493
dc.description.abstractGlycosyltransferase-acceptor activity was demonstrated previously with gametes of Chlamydomonas moewusii and was shown to be enhanced during the mating reaction (McLean and Bosmann, 1975). This investigation is intended to provide some supportive data for the surface localization of sialyltransferase activity and to determine the possibility of hydrolysis of the CMP-sialic acid substrate by surface hydrolases resulting in uptake of the labelled sialic acid. The data indicate that Chlamydomonas cannot utilize sialic acid £or growth in the dark although it is taken up by the cell. Free sialic acid uptake is not enhanced during mating. An excess of free sialic acid did not suppress sialyltransferase activity thus weakening the possibility that CMP-sialic acid, the donor in the reaction, was hydrolyzed resulting in uptake of the free monosaccharide. Trypsinization of gametes before mating significantly reduced the level of activity in the transferase assay. Trypsinization after mating removed nearly all of the label incorporated during the assay. These data support the observation that sialyltransferase-acceptor activity detected on Chlamydomonas gametes is surface-localized and associated with the mating reaction.
dc.subjectSurface Localization
dc.subjectHydrolysis
dc.subjectCell Mating
dc.subjectTrypsinization
dc.subjectTransferase Assay
dc.titleCell Surface Localization of the Sialyltransferase Ectoenzyme System During the Chlamydomonas Mating Reaction
dc.typethesis
refterms.dateFOA2021-09-07T21:02:41Z
dc.description.institutionSUNY Brockport
dc.description.degreelevelMaster of Science (MS)
dc.source.statuspublished
dc.description.publicationtitleBiology Master’s Theses
dc.contributor.organizationThe College at Brockport
dc.languate.isoen_US


Files in this item

Thumbnail
Name:
bio_theses/26/fulltext (1).pdf
Size:
1.876Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record