• Login
    View Item 
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Brockport Biology Master’s Theses
    • View Item
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Brockport Biology Master’s Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SUNY Open Access RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsDepartmentThis CollectionPublication DateAuthorsTitlesSubjectsDepartmentAuthor ProfilesView

    My Account

    LoginRegister

    Campus Communities in SOAR

    Alfred State CollegeBrockportBroomeCantonDownstateDutchessEmpireFarmingdaleFinger LakesFredoniaMaritimeNew PaltzNiagaraOld WestburyOneontaOnondagaOptometryOswegoPlattsburghPurchase CollegePolytechnic InstituteSUNY Office of Workforce Development and Upward MobilitySUNY PressUpstate Medical

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    The Development and Assay of an S-Phase Synchronized Cell System Using Skin Fibroblast Cultures of the Indian Muntjac

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    bio_theses/25/fulltext (1).pdf
    Size:
    2.751Mb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Author
    Cascino, Robert A.
    Keyword
    Autoradiographic Assay
    Hydroxyurea Block-Release
    Synchronized Fusion
    S-Phase Portion
    Date Published
    1977-12-01
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.12648/4492
    Abstract
    An in vitro mammalian cell system which enables direct observation of DNA replication forks is essential to the study and understanding of the mammalian cell cycle S-phase. The prerequisite work of establishing S-phase synchronized cell cultures and an assessment of synchrony levels attained is the object of this study. Skin fibroblasts of the male Indian muntjac line which possesses large chromosomes and a low diploid number were selected for this work. Initial block-release experiments using mitosis as an assay point indicated that 1.5 mM hydroxyurea provided marked levels of synchrony in the absence of detectable cytotoxicity. Premature chromosome condensation patterns of interphase cells fused with mitotic cells indicated a substantially higher S-phase portion of cell populations subjected to hydroxyurea block-release. This was observed in contrast to lower S-phase portions in non-treated controls. These results were supported autoradiographic assays which showed a higher percentage of 3H-thymidine-labeled nuclei in hydroxyurea-treated populations than in controls. It is expected that large quantities of prematurely condensed replicating chromosomes as obtained by this synchronized fusion method would effectively expose sites of DNA replication forks under the electron microscope. This would allow DNA synthesis to be more completely quantified and characterized under normal conditions as well as under conditions which alter this critical phase.
    Collections
    Brockport Biology Master’s Theses

    entitlement

     

    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.