• Login
    View Item 
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Biology Master’s Theses
    • View Item
    •   Home
    • University Colleges
    • SUNY Brockport
    • Theses
    • Biology Master’s Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SUNY Open Access RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsDepartmentThis CollectionPublication DateAuthorsTitlesSubjectsDepartmentAuthor ProfilesView

    My Account

    LoginRegister

    Campus Communities in SOAR

    Alfred State CollegeBrockportBroomeCantonDownstateEmpireFredoniaMaritimeNew PaltzOneontaOptometryOswegoPlattsburghSUNY Polytechnic InstituteSUNY Office of Community Colleges and the Education PipelineSUNY PressUpstate Medical

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    An Investigation of Melanin-concentrating Hormone Receptor Internalization – Or Lack Thereof

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    bio_theses/98/fulltext (1).pdf
    Size:
    2.610Mb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Author
    Moden, Jay I.
    Keyword
    MCH
    Desensitization Mechanisms
    Receptor Internalization
    Mediated Signaling
    Elisa
    Date Published
    2012-01-01
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.12648/4472
    Abstract
    Melanin-concentrating hormone (MCH), a cyclic peptide hormone involved in energy homeostasis, is known to bind to two G protein-coupled receptors (GPCRs) in mammals. These receptors, melanin-concentrating hormone receptor 1 (MCHR1) and melanin-concentrating hormone receptor 2 (MCHR2), have been a popular target for MCH antagonists in an effort to fight the ongoing epidemic of obesity. In the presence of prolonged stimulus it is common for GPCRs to undergo rapid desensitization. However, the desensitization mechanisms of MCHR1 and MCHR2 are as yet poorly understood. This study aims to create epitope-tagged expression vectors to allow for the expression of MCHR1 and MCHR2 in a tissue model. Utilizing a modified cell-based ELISA and fluorescence microscopy, the sequestration of MCHR1 and MCHR2 would be measured after agonist stimulus. Receptor interactions with GRK2 and ?-arrestins would also be measured. The over expression of both MCHR1 and MCHR2 proved to be cytotoxic to BHK570 cells. The overexpression of GRK2, ?-arrestin 1, and ?-arrestin 2 showed a relatively small but statistically significant increase in receptor internalization. Fluorescence microscopy suggests that the interaction between MCHR1 and ?-arrestins were transient in nature. These finding suggest that MCHR1 can be internalized via the clathrin-mediated pathway. It is likely MCH signaling is mediated a cell specific manner based on the cellular expression levels of GRKs and ?-arrestins.
    Collections
    Biology Master’s Theses

    entitlement

     

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.