Mutagenesis at Amino Acid Phosphorylation sites of the Human ERH protein

Abstract

The goal of this project is to successfully create plasmids containing mutant human enhancer rudimentary, e(r) genes. The e(r) gene encodes for the protein, enhancer rudimentary homolog (ERH), which has been shown to promote the progression and survival of certain human cancers. These plasmids containing the mutant human genes will be inserted into the genome of Drosophila melanogaster, the common fruit fly, to study the effects of the mutations on the activity of the protein.