• Characterization of Microplastics using Fourier Transform Infrared Spectroscopy (FTIR)

      Garneau, Danielle; Ashline, Erin (2018)
      Fourier transform infrared (FT-IR) is a spectroscopy technique widely used to analyze polymer profiles of particulate at a chemical level. The goal of this study was to assess the polymer composition of microplastics ingested by aquatic organisms from Lake Champlain. Preliminary results suggest fibers are the most prominent particle type in organisms (N = 482). Among these fibers, the most common plastic polymer was polyester [PET] (14.5%), followed by cellulose [20u ave particle size] (11.1%), alpha-cellulose [99.5% pure] (11.0%), and rayon (8.5%). Fragments were the second most prominent particle type (N = 168) and were commonly polyester [PET] (52%), followed by vinal (9%), polypropylene, isotactic (4%), and rayon (4%). Pellets (N = 14) were primarily vinylidene chlorine [200ppm mhdq] (14.2%) and polyethylene, chlorinated 36% chlorine (14.2%), followed by both vinal (7%), and cellulose nitrate (7%). Films (N = 11) were primarily rayon (27%), poly [methylmethacrylate] (27%), followed by poly [1,4-cyclohexanedimethylene terephthalate] (18%), and polypropylene, isotactic (9%). The least common polymer type found were foams (N = 10) comprised of polyethylene, chlorosulfonated (50%), polyethylene, chlorinated 36% chlorine (40%), and alzon [casein] (10%). Overall, polyester [PET] was more abundant as compared to other plastics and derives from synthetic clothing and food and beverage packaging.
    • Microplastic Biomagnification in Invertebrates, Fish, and Cormorants in Lake Champlain

      Garneau, Danielle; Stewart, James; Walrath, Joshua; Putnam, Alex; Hammer, Chad; VanBrocklin, Hope; Buksa, Brandon; Clune, Alexis (2018)
      The goal of this research was to determine whether microplastics (MP) are uptaken by invertebrates, fish, and Phalacrocorax auritus (double-crested cormorants) resident to Lake Champlain. We did so by quantifying and characterizing (e.g., fragment, fiber, film, foam, pellet) plastic particulate.Wet peroxide oxidation digests were performed on digestive tracts of 506 lake organisms, specifically invertebrates (n = 301), 15 species of fish (n = 190), and Phalacrocorax auritus (double-crested cormorants) (n = 15). Our research indicated that fibers were the were the most common (80.1%) type of particulate found in all organisms, followed by fragments (9.64%), films (6.36%), foam (3.01%), and pellets (Amia calva) contained the greatest average number of plastic particulate (n = 29.67), followed by lake trout (Salvelinus hamaycush) (n = 21.42), and northern pike (Esox lucius) (n = 20.1). Among digested fish, stomachs contained the greatest mean number of MPs (n=5.62), followed by the esophagus (n=5.36) and intestines (n=4.8). These findings suggest biomagnification and/or direct ingestion is occurring in Lake Champlain organisms, as invertebrates, fish, and double-crested cormorants contained on average 0.36, 6.08, and 22.93 microplastic particles.