Browsing Center for Earth and Environmental Science Student Work by Subject "DNA extraction"
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Field and Molecular Survey of Northern and Southern Flying Squirrels in Northern New YorkIn northern New York, there are 2 sympatric species of flying squirrel, the northern (Glaucomys sabrinus) and southern (Glaucomys volans). Recent research suggests that the boundaries between the northern and the southern flying squirrels have been shifting northward, in part due to climate change affecting resource availability. As a result, northern New York would now be the southern terminus for northern, and the northern terminus for the southern flying squirrels. The goal of our research is to develop a molecular survey of the two species that can be compared to morphological measurements made in the field to better confirm species in our area. As weather permits, we are establishing an arboreally mounted trapline and recovering scat, hair, and other tissue samples for DNA extraction, polymerization chain reaction (PCR), and restriction digests to determine species. The molecular assay has been optimized; however the harsh winter has limited our trapping success for tissue collection in the field. Determining frequency of capture will assist in predicting where the boundary of these species lies in Clinton County.