• The impact of mosquito salivary factors on Chikungunya virus transmission

      Thangamani, Saravanan; Esterly, Allen (2022-08-12)
      Vector-borne diseases affect an estimated 3.9 billion people in over 128 countries annually (WHO, 2017b). Mosquito-borne viruses, such as Chikungunya virus (CHIKV), are transmitted to human hosts through the saliva of female blood-feeding mosquitoes. During blood-feeding, salivary molecules enhance blood-feeding efficiency, dampen specific inflammatory signals, and enhance virus infection and dissemination. Skin is the interface where mosquito salivary molecules and viruses interact with the human immune system. Our long-term goal is to understand the impact of mosquito saliva on the skin and the role of salivary molecules in potentiating virus infection. This thesis aims to model the human skin bite site, identify mosquito salivary molecules present during virus infection, and investigate their impact on virus replication. We developed a human skin arbovirus infection model that was viable for four days ex vivo (Esterly et al., 2022a). Arbovirus infection in human skin ex vivo allowed flavivirus and alphavirus replication and dissemination. Using this model, we will investigate mosquito salivary factors and their impact on virus transmission. Next, we identified mosquito salivary factors highly expressed in Ae. aegypti salivary glands during CHIKV infection. We hypothesize that salivary proteins that contain secretion signals and are found to be highly expressed during CHIKV infection are delivered to the bite site during mosquito feeding. Through a combination of RNA-seq and miRNA-seq analysis, we identified several salivary factors, protein and small non-coding RNAs, which warrant further investigation. Lastly, we assessed the impact of identified and recombinantly expressed proteins or specific small RNA inhibitors on CHIKV replication in vitro relative to Ae. aegypti salivary gland extract (SGE). We found that both protein and small RNAs can influence CHIKV replication in a significant manner; however, the effect observed by mosquito saliva is a combination of multiple factors and will require more investigation to characterize fully. In summary, mosquito salivary factors work in concert to enhance virus replication and dissemination. Although individual factors may not recapitulate the total effect of SGE, the discovery and isolation of these factors further our understanding of arbovirus transmission. These studies highlight the complexity of mosquito salivary secretion and further our knowledge of mosquito-borne virus transmission. The following thesis will describe our most recent findings and contribution to understanding mosquito saliva's role during arbovirus transmission.
    • Impact of Psychometrically Defined Deficits of Executive Functioning in Adults With Attention Deficit Hyperactivity Disorder

      Biederman, Joseph; Petty, Carter; Fried, Ronna; Fontanella, Jessie; Doyle, Alysa E.; Seidman, Larry J.; Faraone, Stephen V. (American Psychiatric Association Publishing, 2006-10)
      Objective: The association between deficits in executive functioning and functional outcomes was examined among adults with attention deficit hyperactivity disorder (ADHD). Method: Subjects were adults who did (N=213) and did not (N=145) meet DSMIV criteria for ADHD. The authors defined having deficits in executive functioning as having at least two measures of executive functioning with scores 1.5 standard deviations below those of matched comparison subjects. Results: Significantly more adults with ADHD had deficits of executive functioning than comparison subjects. Deficits of executive functioning were associated with lower academic achievement, irrespective of ADHD status. Subjects with ADHD with deficits of executive functioning had a significantly lower socioeconomic status and a significant functional morbidity beyond the diagnosis of ADHD alone. Conclusions: Psychometrically defined deficits of executive functioning may help identify a subgroup of adults with ADHD at high risk for occupational and academic underachievement. More efforts are needed to identify cost-effective approaches to screen individuals with ADHD for deficits of executive functioning.
    • The Impact of Staff Development on Middle School Technology Integration

      Scalisi, Regina (2005-05-01)
      This case study describes the impact of technology staff development in two Central New York Middle Schools. The staff development program was implemented under the federally funded Enhancing Education Through Technology grant. Teachers and students were studied during a seven-month period as they learned to effectively use computers, Internet resources and available software with their curricula and instruction. The conditions of Change Theory were applied to the study as they relate to the adoption of technology integration.
    • Impact of Tic Disorders on ADHD Outcome Across the Life Cycle: Findings From a Large Group of Adults With and Without ADHD

      Spencer, Thomas J.; Biederman, Joseph; Faraone, Stephen V.; Mick, Eric; Coffey, Barbara; Geller, Daniel; Kagan, Jake; Bearman, Sarah Kate; Wilens, Timothy (American Psychiatric Association Publishing, 2001-04)
      Objective: The impact of tic disorders on the outcome of attention deficit hyperactivity disorder (ADHD) remains a subject of high scientific and clinical interest. To evaluate the impact of comorbid ADHD and tic disorders from a lifespan perspective, the authors systematically examined data from adults with and without ADHD. Method: They comprehensively evaluated 312 consecutively referred adults with ADHD and 252 comparison subjects without ADHD. Tic disorders were characterized along with a wide range of neuropsychiatric correlates, including other comorbid disorders as well as indexes of function in the domains of school, cognition, and interpersonal functioning. Results: A significantly greater proportion of adults with ADHD (12%) than those without ADHD (4%) had tic disorders. Tic disorders followed a mostly remitting course and had little impact on functional capacities. In addition, tic disorders were not associated with stimulant use. Conclusions: These findings in adults with ADHD confirm and extend previous findings in young subjects with ADHD, documenting that although individuals with ADHD are at greater risk for tic disorders, the presence of tic disorders has a limited impact on ADHD outcome.
    • The Influence of College Web Sites on the College Selection Process

      Basi Raab, Maryrose (2005-12-01)
      This case study examines the use and influence of college web sites by high school seniors as they move through the college selection process through interviews, directed observations, and focus groups. It discovers and describes the different ways that web sites are used by this bounded group. As a part of the study, the re-designed web site at SUNY Institute of Technology is critiqued and analyzed by respondents. Enhancements are proposed based on the research to increase the effectiveness of the site in its appeal to prospective high school students. The theories of Human-Centered Design and Contextual Design are described and applied to this case study.
    • Influence of Gender on Attention Deficit Hyperactivity Disorder in Children Referred to a Psychiatric Clinic

      Biederman, Joseph; Mick, Eric; Faraone, Stephen V.; Braaten, Ellen; Doyle, Alysa; Spencer, Thomas; Wilens, Timothy E.; Frazier, Elizabeth; Johnson, Mary Ann (American Psychiatric Association Publishing, 2002-01)
      Objective: The substantial discrepancy in the male-to-female ratio between clinic-referred (10 to 1) and community (3 to 1) samples of children with attention deficit hyperactivity disorder (ADHD) suggests that gender differences may be operant in the phenotypic expression of ADHD. In this study the authors systematically examined the impact of gender on the clinical features of ADHD in a group of children referred to a clinic. Method: The study included 140 boys and 140 girls with ADHD and 120 boys and 122 girls without ADHD as comparison subjects. All subjects were systematically assessed with structured diagnostic interviews and neuropsychological batteries for subtypes of ADHD as well as emotional, school, intellectual, interpersonal, and family functioning. Results: Girls with ADHD were more likely than boys to have the predominantly inattentive type of ADHD, less likely to have a learning disability, and less likely to manifest problems in school or in their spare time. In addition, girls with ADHD were at less risk for comorbid major depression, conduct disorder, and oppositional defiant disorder than boys with ADHD. A statistically significant gender-by-ADHD interaction was identified for comorbid substance use disorders as well. Conclusions: The lower likelihood for girls to manifest psychiatric, cognitive, and functional impairment than boys could result in gender-based referral bias unfavorable to girls with ADHD
    • The influence of genes on “positive valence systems” constructs: A systematic review

      Hess, Jonathan L.; Kawaguchi, Daniel M.; Wagner, Kayla E.; Faraone, Stephen V.; Glatt, Stephen J. (Wiley, 2015-09-14)
      The Research Domain Criteria (RDoC) address three types of aggression: frustrative non-reward, defensive aggression and offensive/proactive aggression. This review sought to present the evidence for genetic underpinnings of aggression and to determine to what degree prior studies have examined phenotypes that fit into the RDoC framework. Although the constructs of defensive and offensive aggression have been widely used in the animal genetics literature, the human literature is mostly agnostic with regard to all the RDoC constructs. We know from twin studies that about half the variance in behavior may be explained by genetic risk factors. This is true for both dimensional, trait-like, measures of aggression and categorical definitions of psychopathology. The non-shared environment seems to have a moderate influence with the effects of shared environment being unclear. Human molecular genetic studies of aggression are in an early stage. The most promising candidates are in the dopaminergic and serotonergic systems along with hormonal regulators. Genome-wide association studies have not yet achieved genome-wide significance, but current samples are too small to detect variants having the small effects one would expect for a complex disorder. The strongest molecular evidence for a genetic basis for aggression comes from animal models comparing aggressive and non-aggressive strains or documenting the effects of gene knockouts. Although we have learned much from these prior studies, future studies should improve the measurement of aggression by using a systematic method of measurement such as that proposed by the RDoC initiative. © 2015 Wiley Periodicals, Inc.
    • Influence of Parental SUD and ADHD on ADHD in their Offspring: Preliminary Results from a Pilot-controlled Family Study

      Wilens, Timothy E.; Hahesy, Amy L.; Biederman, Joseph; Bredin, Elizabeth; Tanguay, Sarah; Kwon, Anne; Faraone, Stephen V. (Wiley, 2005-03)
      As part of a pilot-controlled family-based study of the children of parents with and without substance use disorders (SUD), the influence of parental SUD and ADHD on the risk for ADHD in offspring was evaluated. Using structured psychiatric interviews, 96 families (183 youth; mean age 11.6 years) were assessed. To evaluate the effect of parental ADHD and SUD, the offspring were stratified into four groups based on parental status: children of parents with neither ADHD nor SUD, children of parents with SUD only, children of parents with ADHD only, and children of parents with both ADHD and SUD. Using generalized estimating equation models, parental SUD and ADHD were used to predict ADHD in the offspring. The rate of children with ADHD increased among children of parents with neither disorder (3%), children of parents with SUD (13%), children of parents with ADHD (25%), and children of parents with both ADHD and SUD (50%) (p ¼ :001). Children of parents with ADHD or ADHD plus SUD were more likely to have ADHD in comparison to children of parents with neither diagnosis (p < 0:05). Children of parents with ADHD plus SUD were at greater risk of ADHD in comparison to children of parents with SUD only (p ¼ 0:01). Despite the small sample size, the results of this study seem to suggest that the offspring of SUD or ADHD parents are at elevated risk for ADHD compared to controls. The offspring of parents with both ADHD and SUD appear to be at the highest risk for ADHD, highlighting the need for careful screening of this group of youth for ADHD. Replication studies clarifying the nature and strength of the association are necessary.
    • The Influence of the DVD format on the College Selection Process

      Lanagan, Douglas F (2011-01-01)
      This case study centers on the role of the DVD format in regards to the decision making process about selecting a college. This study centers on the possible role that a DVD could play in that selection process, and what features an effective DVD might include in order to play a role in that decision. A Pilot DVD was produced for recruitment purposes in the Radio-Television Broadcasting program at a small upstate New York community college. Qualitative interviews were conducted with administrators, Admissions staff, prospective, current, and future students.Surveys were also conducted, both in person and online. The production process and distribution of the DVD is described, and the role of the DVD in the decision making process for selecting a college was examined. The evolution of Web 2.0 technologies was also taken into account. The research indicates that the DVD format was useful to students in their decision making process regarding enrolling in the program. It was also determined in this case study that the impact of the DVD alone, when considered against all other media used in college recruitment and decision making, was difficult to ascertain.
    • Infographics - Migrating from paper methods to electronic using an enterprise system - Jenzabar EX

      Stam, Kathryn; Thesis Advisor; Lizardi, Ryan; Second Reader; Psarudakis, Robert J. (2021-05)
      SUNY Sullivan has been facing challenges of declining enrollment since the start of the recession in 2008. For the most part the college seems to go through a roller-coaster ride of increasing but steadily declining enrollment spirals ever since. One of our key survival strategies is adaptation to change. Adaptations with programs, delivery methods in the way we offer programs and courses. We tend to be on the cutting edge of technology as it evolves and we adapt to new challenges as long as our funds from SUNY are available. In this paper I will identify some of the gaps in processes that are simply not working. I will look at the theories behind the changes and challenges that are associated with modern adaptations in addition to the reasons why we must change. In this respect I will be looking at our old processes such as paper forms and face to face contact. We are encountering a new world in response to the COVID-19 pandemic. COVID-19 causes all of our in-person activities to be remote. Currently, we are going through our Middle States self-study evaluation. "The Middle States Commission on Higher Education is a voluntary, non-governmental, institutional membership association currently serving higher education institutions in Delaware, the District of Columbia, Maryland, New Jersey, New York, Pennsylvania, Puerto Rico, the Virgin Islands, and any other geographic areas in which the Commission elects to conduct accrediting activities within the United States." (MSCHE, website). Looking at what we do as an institution, we found that we have many "gaps" that need to be filled in. Nothing has proven this more than COVID-19.
    • Information Design and Technology

      Ossowski, Candice (2004-05-01)
      This case study analyzes the effects of transforming a static HTML-based website into a dynamic database-driven one. As part of the study, a small computer company’s static website was redesigned, making it dynamic and database-driven. The theory of Human-Centered Design is applied to a real-world situation and the nine human-centered characteristics are analyzed as they relate to static and dynamic websites
    • Informativeness of Self-Reports of ADHD Symptoms in Monitoring Response to Stimulant Treatment in Clinically Referred Adults With ADHD

      Biederman, Joseph; Fitzgerald, Maura; Spencer, Thomas J.; Adler, Lenard A.; Abrams, Jessica; Biederman, Itai; Faraone, Stephen V. (SAGE Publications, 2018-05-26)
      To investigate the informativeness of self-reports of ADHD symptoms in adults with ADHD in the clinical setting. Method: Subjects were clinically referred adults aged 19 years to 67 years of age of both sexes (N = 54). All subjects were on stable doses of stimulant and were considered responders to treatment. ADHD symptoms were assessed using the ADHD Investigator Symptom Rating Scale (AISRS) and the ADHD Self-Report Scale (ASRS). Spearman’s rank correlations were used to assess the correlations between clinician-assessed ADHD and patients’ self-reports. Results: Spearman’s rank correlation analysis found evidence of a strong, positive association between total scores on the AISRS and the ASRS (rs = .65, df = 52, p < .001). Conclusion: Results have important implications for the management and monitoring of treatment response in the clinical setting through patients’ self-report.(J. of Att. Dis. 2020; 24(3) 420-424)
    • Integrating Information and Communication Tools Effective Strategies for the Classroom

      Phan, Thuc (2013-05-01)
      This project presents effective strategies for integrating information and communication tools (ICTs) within a class. Three major goals that suggest effective implementation of ICTs are, enhancing education by meeting the needs of different learners, facilitating higher levels of learning, and increasing computer literacy skills. Findings from scholarly articles provided a foundation of methods, principles, and strategies in meeting these goals using ICTs. Implementation of integrating ICTs will be shown with an example computer technology course to demonstrate effective strategies. This example course will be used to present effective strategies that are supported by findings from previous research and will include example learning modules located on a learning management system and routine procedures. The content within the example course will revolve around teaching students computer applications though this subject is not relevant towards meeting any goals for effective implementation if integrating ICTs. This is to suggest that another subject, such as science, can be replaced with teaching computer technology. The main focus within this example course should be on the tools, methods, and procedures of teaching and learning with ICTs and how they are used to enhance the learning environment of this course. The ICTs used in meeting the goals of meeting the needs of different learners, facilitating high levels of learning, and increasing computer literacy skills are Schoology.com, Google Docs, Prezi, and Apple Remote Desktop. These ICTs where chosen based on findings from previous research, observation of trending applications, effectiveness of communication and information management, and potential relevance for the future uses.
    • An Inventory Management App in Salesforce

      Chennamaneni, Rahul Madhava Rao; Chiang, Chen-Fu; Adviser; Novillo, Jorge; Reviewer; Rezk, Mohamed, Reviewer (2016-12-01)
      Salesforce is a cloud-based customer relationship management (CRM) software that accelerates business relationships and can transform the working lives of the team. Marc Benioff developed it in the late 1990s and now it has been announced as the world's most innovative company for six consecutive years by Forbes Magazine [1]. Unlike traditional CRM software, Salesforce is an internet service. It is available with just a sign-up and logs in through a browser, and it is immediately available. It is based on cloud computing, where the customers, without the need of installing any traditional software, can access the cloud, i.e., through the internet, for their business needs [2]. Inventory Management (IM) is the method of controlling and supervising the storage, utilization and ordering of components that an organization can track of their items it sells. It is the act of controlling and administering the quantities of products in the sale. For a business, an inventory is the main asset which represents an investment by the owner until the item is sold [3]. To demonstrate its functionalities of Salesforce, I created an application for inventory management. Here, In the inventory management, there are two parts: one is administration part, and another part is customer portal. The administrator manages the inventory and store operations, and the customer buys the products in the inventory through customer portal.
    • Investigating the Role of Paxillin in Mammary Gland Morphogenesis and Breast Cancer Progression

      Turner, Christopher; Xu, Weiyi (2020)
      Breast cancer is one of the most invasive cancers among women. Understanding the mechanisms contributing to breast cancer progression may identify potential ways to prevent and cure the disease. Meanwhile, mammary gland morphogenesis shares similar mechanisms to allow the ducts to invade and occupy the fat pad. Thus, it is equally important to investigate the normal mammary gland development as breast tumor progression. Paxillin, as a focal adhesion scaffold protein, has previously been implicated in multiple types of cancer cell migration and invasion through its role in cell- ECM signaling. Herein, I utilized a novel paxillin conditional knockout mouse model and paxillin knockout mouse crossed with PyMT breast tumor mouse model to show that paxillin is critical for both mammary gland morphogenesis and breast tumor progression. In Chapter 2, by evaluating the developing mammary gland morphology with immunohistochemistry and the three-dimensional cultured mammary organoids and acini, a critical role of paxillin was shown in facilitating apical-basal polarity formation in the luminal epithelial cells in part, through its control of HDAC6 activity and associated microtubule acetylation. Correct polarization and columnar shape of the epithelial cells potentially contributes to lumen formation and branching of the ducts. Investigation in Chapter 3 highlights a crucial role of paxillin in breast cancer invasion and distant organ metastasis, but did not affect the primary tumor growth rate. Further analysis revealed that paxillin is required for the endocytosis and recycling of E- cadherin, which is important for the maintenance of Adherens junction equilibrium during cancer cell collective migration. This thesis characterizes the roles for paxillin in mammary gland morphogenesis and breast cancer progression and reveals the importance of paxillin-dependent apical trafficking in normal epithelial cells, and E-cadherin trafficking in collective migrating tumor cells. Together, this work highlights a trafficking-dependent mechanism for paxillin during both physiologic and pathologic processes in the mammary gland.