Browsing Senior Honors Theses by Subject "African Sleeping Sickness"
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Arginine Methylation of TbLpn, a Trypanosome Lipin Homologue, by TbPRMT EnzymesPhospholipids biosynthesis, particularly phosphatidylcholine (PC) and phosphatidylethanolamine (PE) plays a major role in the survival of T.brucei. Of great importance is the fact that, as opposed to other parasitic organisms, trypanosomes synthesize phospholipids de novo. Although the pathways for phospholipids biosynthesis have not been very well characterized, recent data have helped to better understand how trypanosomes are able to assemble phospholipids. Previous work in our lab has shown that a protein, termed TbLpn, is a phosphatidic acid phosphatase potentially involved in phospholipid biosynthesis in T.brucei. In addition, TbLpn contains methylated arginine residues and interacts with T.brucei major Protein Arginine Methyltransferases, TbPRMT7. The major focus of my project is to identify the effect of TbLpn methylation by TbPRMT7 on its enzymatic activity and cellular localization.
Disruption of TbLpn Expression in T. brucei by RNA InterferenceTrypanosoma brucei, the causative agent of human African trypanosomiasis, also known as African sleeping sickness, expresses a protein known as TbLpn which plays a critical role in arginine methylation and phospholipid synthesis within T. brucei. In order to determine the effect and potential medical benefits of downregulated TbLpn expression in T. brucei, RNA interference was used by transfecting T. brucei with plasmid DNA through electroporation. After conducting this experimental protocol, T. brucei was unable to be grown successfully in media following transfection and the effect of downregulated TbLpn in T. brucei requires further investigation in order to determine its potential for developing new drug targets and treatment methods for human African trypanosomiasis. Experimental protocol changes and repetition and verification of the validity of current protocols could lead the way for successful growth of T. brucei with downregulated TbLpn.