Browsing Biology Master’s Theses by Author "Fried, Barry"
recA-Independence of Residual Dimer Excision in a recI.152 Mutant of Esherichia C01.1 K-12Rothman, Robert H.; Fried, Barry; The College at Brockport (1982-06-01)Wild-type strains of E. coli possess both short and long patch repair mechanisms to correct UV-induced pyrimidine dimers. Short patch repair is the predominant mode, and long patch repair is recA+ -dependent, requires de novo protein synthesis, and is UV-inducible. Excision repair in a recI.152 mutant is characterized by slow dimer excision and the exhibition of primarily large patches. Residual excision repair in the recI.152 mutant was studied by introducing a recA.56 mutation into the recI.152 strain. Analysis of cell survival, host-cell reactivation, Weigle-reactivation, and the rate of dimer release in both the recL152 single mutant and the recA56 recL152 double mutant reveals that residual excision repair detected in the recL152 strain is recA+ -independent and non-inducible. These data indicate that long patch repair replication in the recL152 mutant is not the same as the long patches seen in wild-type cells, and possibly represents altered regulation of the more important short patch system.