Expression and Purification of Complement Factor H (CFH) to Understand Bridge Between Coagulation and Inflammation

Abstract

Complement Factor H (CFH) is a protein that regulates an innate immune defense and prevents damage to self-tissue by inactivating complement component C3b. To characterize interactions between CFH and other plasma proteins, CFH was expressed from Pichia pastoris and then purified by ion exchange chromatography and size exclusion.