Investigating the Function of Enzyme 2014

Bailine, Ryan; Kuhn, Derek; Koeppe, Julia R.

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Author

Bailine, Ryan
Kuhn, Derek
Koeppe, Julia R.

Date Published

2021

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URI

http://hdl.handle.net/20.500.12648/15401

Abstract

Enzyme 2O14 is a protein of unknown function, but is hypothesized to be an esterase. We investigated the function of this enzyme using a chromogenic ester substrate, p-nitrophenyl acetate (PNPA). The product from the hydrolysis of PNPA is yellow, allowing for the measurement of absorbance as a function of activity. Enzyme 2O14 was shown to hydrolyze the ester substrate, indicated by the increase in absorbance as a result of the formation of the yellow product.

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