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dc.contributor.authorHassan, Md Zakiul
dc.contributor.authorSturm-Ramirez, Katharine
dc.contributor.authorIslam, Md Saiful
dc.contributor.authorAfreen, Sadia
dc.contributor.authorRahman, Mohammed Ziaur
dc.contributor.authorKafi, Mohammad Abdullah Heel
dc.contributor.authorChowdhury, Sukanta
dc.contributor.authorKhan, Salah Uddin
dc.contributor.authorRahman, Mustafizur
dc.contributor.authorNasreen, Sharifa
dc.contributor.authorDavis, C. Todd
dc.contributor.authorLevine, Min Z.
dc.contributor.authorRahman, Mahmudur
dc.contributor.authorLuby, Stephen P.
dc.contributor.authorAzziz-Baumgartner, Eduardo
dc.contributor.authorIuliano, A.D.
dc.contributor.authorUyeki, Timothy M.
dc.contributor.authorGurley, Emily S.
dc.date.accessioned2024-06-26T19:20:15Z
dc.date.available2024-06-26T19:20:15Z
dc.date.issued2023-11
dc.identifier.citationHassan MZ, Sturm-Ramirez K, Islam MS, Afreen S, Rahman MZ, Kafi MAH, Chowdhury S, Khan SU, Rahman M, Nasreen S, Davis CT, Levine MZ, Rahman M, Luby SP, Azziz-Baumgartner E, Iuliano AD, Uyeki TM, Gurley ES. Interpretation of molecular detection of avian influenza A virus in respiratory specimens collected from live bird market workers in Dhaka, Bangladesh: infection or contamination? Int J Infect Dis. 2023 Nov;136:22-28. doi: 10.1016/j.ijid.2023.08.020. Epub 2023 Aug 29. PMID: 37652093.en_US
dc.identifier.issn1201-9712
dc.identifier.doi10.1016/j.ijid.2023.08.020
dc.identifier.pmid37652093
dc.identifier.piiS1201971223007063
dc.identifier.urihttp://hdl.handle.net/20.500.12648/14986
dc.description.abstractObjectives: Interpreting real-time reverse transcription-polymerase chain reaction (rRT-PCR) results for human avian influenza A virus (AIV) detection in contaminated settings like live bird markets (LBMs) without serology or viral culture poses a challenge. Methods: During February-March 2012 and November 2012-February 2013, we screened workers at nine LBMs in Dhaka, Bangladesh, to confirm molecular detections of AIV RNA in respiratory specimens with serology. We tested nasopharyngeal (NP) and throat swabs from workers with influenza-like illness (ILI) and NP, throat, and arm swabs from asymptomatic workers for influenza virus by rRT-PCR and sera for seroconversion and antibodies against HPAI A(H5N1) and A(H9N2) viruses. Results: Among 1273 ILI cases, 34 (2.6%) had A(H5), 56 (4%) had A(H9), and six (0.4%) had both A(H5) and A(H9) detected by rRT-PCR. Of 192 asymptomatic workers, A(H5) was detected in eight (4%) NP and 38 (20%) arm swabs. Of 28 ILI cases with A(H5) or A(H9) detected, none had evidence of seroconversion, but one (3.5%) and 12 (43%) were seropositive for A(H5) and A(H9), respectively. Conclusion: Detection of AIV RNA in respiratory specimens from symptomatic and asymptomatic LBM workers without evidence of seroconversion or virus isolation suggests environmental contamination, emphasizing caution in interpreting rRT-PCR results in high viral load settings.en_US
dc.description.sponsorshipMinistry of Science and Innovation, New Zealanden_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.relation.urlhttps://www.ijidonline.com/article/S1201-9712(23)00706-3/fulltexten_US
dc.rights© 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectA(H5N1) diagnosisen_US
dc.subjectAvian influenza A virusen_US
dc.subjectHPAI A(H5N1)en_US
dc.subjectLive bird marketen_US
dc.subjectViral contaminationen_US
dc.subjectrRT-PCR resultsen_US
dc.titleInterpretation of molecular detection of avian influenza A virus in respiratory specimens collected from live bird market workers in Dhaka, Bangladesh: infection or contamination?en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleInternational Journal of Infectious Diseasesen_US
dc.source.volume136
dc.source.beginpage22
dc.source.endpage28
dc.description.versionVoRen_US
refterms.dateFOA2024-06-26T19:20:17Z
dc.description.institutionSUNY Downstateen_US
dc.description.departmentEpidemiology and Biostatisticsen_US
dc.description.degreelevelN/Aen_US


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