Chlamydia pneumoniae-induced tumour necrosis factor alpha responses are lower in children with asthma compared with non-asthma.
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Author
Smith-Norowitz, Tamar AnneChotikanatis, Kobkul
Weaver, Diana
Ditkowsky, Jared
Norowitz, Yitzchok Meir
Hammerschlag, Margaret R
Joks, Rauno
Kohlhoff, Stephan
Journal title
BMJ open respiratory researchDate Published
2018-05-05Publication Volume
5Publication Issue
1Publication Begin page
e000239
Metadata
Show full item recordAbstract
respiratory tract infection has been implicated in the pathogenesis of reactive airway disease and asthma. Innate cytokine responses that are protective of infection with intracellular pathogens may be impaired in patients with asthma. Tumour necrosis factor alpha (TNF-α) is a cytokine related to functions of monocytes and may inhibit infection. We investigated TNF-α responses in -infected peripheral blood mononuclear cells (PBMCs) in patients with asthma and non-asthma, and whether ciprofloxacin, azithromycin or doxycycline affects TNF-α responses.PBMC (1.5×10) from paediatric patients with asthma (n=19) and non-asthmatic controls (n=6) were infected or mock infected for 1 hour with or without AR-39 at a multiplicity of infection=0.1, and cultured+ciprofloxacin, azithromycin or doxycycline (0.1 ug/mL) for 48 hours. TNF-α levels were measured in supernatants by ELISA.
When PBMC from patients with asthma were infected with , levels of TNF-α were significantly lower than in subjects without asthma (48 hours) (5.5±5.6, 38.4±53.7; p=0.0113). However, baseline responses (no infection with ) were similar in asthma and non-asthma (1.0±1.7, 1.1±1.2; p=0.89). When PBMC frompatiens with asthma were infected with +ciprofloxacin, azithromycin or doxycycline, TNF-α levels increased (25%-45%); this affect was not observed in PBMC from patients without asthma.
We identified differences in the quantity of TNF-α produced by infected PBMC in asthma compared with non-asthma.
Citation
Smith-Norowitz TA, Chotikanatis K, Weaver D, Ditkowsky J, Norowitz YM, Hammerschlag MR, Joks R, Kohlhoff S. Chlamydia pneumoniae-induced tumour necrosis factor alpha responses are lower in children with asthma compared with non-asthma. BMJ Open Respir Res. 2018 May 5;5(1):e000239. doi: 10.1136/bmjresp-2017-000239. PMID: 29755754; PMCID: PMC5942456.DOI
10.1136/bmjresp-2017-000239ae974a485f413a2113503eed53cd6c53
10.1136/bmjresp-2017-000239
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International
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