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dc.contributor.authorKumar, Swati
dc.contributor.authorSmith-Norowitz, Tamar A
dc.contributor.authorKohlhoff, Stephan
dc.contributor.authorApfalter, Petra
dc.contributor.authorRoblin, Patricia
dc.contributor.authorKutlin, Andrei
dc.contributor.authorHarkema, Jack
dc.contributor.authorNg, Sheung P
dc.contributor.authorDoherty-Lyons, Shannon
dc.contributor.authorZelikoff, Judith T
dc.contributor.authorHammerschlag, Margaret R
dc.date.accessioned2023-07-07T16:31:10Z
dc.date.available2023-07-07T16:31:10Z
dc.date.issued2016
dc.identifier.citationKumar S, Smith-Norowitz TA, Kohlhoff S, Apfalter P, Roblin P, Kutlin A, Harkema J, Ng SP, Doherty-Lyons S, Zelikoff JT, Hammerschlag MR. Exposure to cigarette smoke and Chlamydia pneumoniae infection in mice: Effect on infectious burden, systemic dissemination and cytokine responses: A pilot study. J Immunotoxicol. 2016;13(1):77-83. doi: 10.3109/1547691X.2015.1006346. Epub 2015 Feb 2. PMID: 25640695.en_US
dc.identifier.eissn1547-6901
dc.identifier.doi10.3109/1547691X.2015.1006346
dc.identifier.pmid25640695
dc.identifier.urihttp://hdl.handle.net/20.500.12648/10398
dc.description.abstractCigarette smoke exposure has been considered a risk factor for infection with Chlamydia pneumoniae. C. pneumoniae infection is associated with respiratory tract infection and chronic respiratory disease, which is a serious public health concern. To determine whether prior exposure to cigarette smoke worsens C. pneumoniae infection (specifically, increases infectious burden and systemic dissemination) as well as alters cytokine responses in mice, adult female C57BL/6 mice were exposed to either filtered air (FA) or mainstream cigarette smoke (MCS) (15 mg/m(3), total suspended particulates) for 5 days/week for 2 weeks and then infected with C. pneumoniae (10(5) IFU) via intratracheal instillation. Mice were euthanized on Days 7, 14 or 26 post-infection (p.i.). Chlamydial burdens in the lungs and spleen were quantified by quantitative PCR (qPCR) and histologic analyses were performed; cytokine levels (TNFα, IL-4, IFNγ) in bronchoalveolar lavage fluid and serum were assayed by enzyme-linked immunosorbent assay (ELISA). The results indicated that: (1) mice exposed to either FA or MCS had similar chlamydial burdens in the lungs and spleen on Days 14 and 26 p.i.; (2) proximal and distal airway inflammation was observed on Day 14 p.i. in both FA and MCS mice, but persisted in MCS mice until Day 26 p.i.; FA exposed mice demonstrated resolution of distal airway inflammation; and (3) MCS mice displayed higher serum levels of IFNγ and IL-4 on Day 26 p.i. These findings indicate that exposure of mice to MCS (at a concentration equivalent to smoking < 1 pack cigarettes/day) led to greater C. pneumoniae-induced inflammation, as indicated by prolonged inflammatory changes.
dc.language.isoenen_US
dc.relation.urlhttps://www.tandfonline.com/doi/full/10.3109/1547691X.2015.1006346en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectChlamydia pneumoniaeen_US
dc.subjectcigarette smokeen_US
dc.subjectcytokinesen_US
dc.subjectmiceen_US
dc.titleExposure to cigarette smoke and Chlamydia pneumoniae infection in mice: Effect on infectious burden, systemic dissemination and cytokine responses: A pilot study.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleJournal of immunotoxicologyen_US
dc.source.volume13
dc.source.issue1
dc.source.beginpage77
dc.source.endpage83
dc.source.countryEngland
dc.description.versionVoRen_US
refterms.dateFOA2023-07-07T16:31:10Z
html.description.abstractCigarette smoke exposure has been considered a risk factor for infection with Chlamydia pneumoniae. C. pneumoniae infection is associated with respiratory tract infection and chronic respiratory disease, which is a serious public health concern. To determine whether prior exposure to cigarette smoke worsens C. pneumoniae infection (specifically, increases infectious burden and systemic dissemination) as well as alters cytokine responses in mice, adult female C57BL/6 mice were exposed to either filtered air (FA) or mainstream cigarette smoke (MCS) (15 mg/m(3), total suspended particulates) for 5 days/week for 2 weeks and then infected with C. pneumoniae (10(5) IFU) via intratracheal instillation. Mice were euthanized on Days 7, 14 or 26 post-infection (p.i.). Chlamydial burdens in the lungs and spleen were quantified by quantitative PCR (qPCR) and histologic analyses were performed; cytokine levels (TNFα, IL-4, IFNγ) in bronchoalveolar lavage fluid and serum were assayed by enzyme-linked immunosorbent assay (ELISA). The results indicated that: (1) mice exposed to either FA or MCS had similar chlamydial burdens in the lungs and spleen on Days 14 and 26 p.i.; (2) proximal and distal airway inflammation was observed on Day 14 p.i. in both FA and MCS mice, but persisted in MCS mice until Day 26 p.i.; FA exposed mice demonstrated resolution of distal airway inflammation; and (3) MCS mice displayed higher serum levels of IFNγ and IL-4 on Day 26 p.i. These findings indicate that exposure of mice to MCS (at a concentration equivalent to smoking < 1 pack cigarettes/day) led to greater C. pneumoniae-induced inflammation, as indicated by prolonged inflammatory changes.
dc.description.institutionSUNY Downstateen_US
dc.description.departmentPediatricsen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalJournal of immunotoxicology
dc.identifier.issue1en_US


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