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dc.contributor.authorChernesky, Max
dc.contributor.authorSmieja, Marek
dc.contributor.authorSchachter, Julius
dc.contributor.authorSummersgill, James
dc.contributor.authorSchindler, Laura
dc.contributor.authorSolomon, Natalie
dc.contributor.authorCampbell, Karen
dc.contributor.authorCampbell, LeeAnn
dc.contributor.authorCappuccio, Alison
dc.contributor.authorGaydos, Charlotte
dc.contributor.authorChong, Sylvia
dc.contributor.authorMoncada, Jeanne
dc.contributor.authorPhillips, Jack
dc.contributor.authorJang, Dan
dc.contributor.authorWood, Billie Jo
dc.contributor.authorPetrich, Astrid
dc.contributor.authorHammerschlag, Margaret
dc.contributor.authorCerney, Mike
dc.contributor.authorMahony, James
dc.date.accessioned2023-07-05T15:56:18Z
dc.date.available2023-07-05T15:56:18Z
dc.date.issued2002-07
dc.identifier.citationChernesky M, Smieja M, Schachter J, Summersgill J, Schindler L, Solomon N, Campbell K, Campbell L, Cappuccio A, Gaydos C, Chong S, Moncada J, Phillips J, Jang D, Wood BJ, Petrich A, Hammerschlag M, Cerney M, Mahony J. Comparison of an industry-derived LCx Chlamydia pneumoniae PCR research kit to in-house assays performed in five laboratories. J Clin Microbiol. 2002 Jul;40(7):2357-62. doi: 10.1128/JCM.40.7.2357-2362.2002. PMID: 12089248; PMCID: PMC120594.en_US
dc.identifier.issn0095-1137
dc.identifier.pmid12089248
dc.identifier.urihttp://hdl.handle.net/20.500.12648/10364
dc.description.abstractIn a multicenter comparison of PCR assays utilizing 120 quantitated samples of 16 Chlamydia pneumoniae isolates, an LCx research-use-only (RUO) PCR developed by Abbott Laboratories demonstrated 100% sensitivity on 48 samples with >1 copy of DNA per microl of specimen. The sensitivities of five in-house PCR assays ranged from 54 to 94% for the same samples. All six assays showed decreased sensitivities as the DNA copy numbers of the samples decreased. Overall, sensitivities ranged from 68% for the LCx PCR assay to 29% for one of the in-house tests. The LCx RUO PCR and three of the five in-house PCR tests reported no false positives with the 24 negative samples. Increasing the number of replicates tested increased the sensitivities of all of the assays, including the LCx PCR. The LCx RUO assay showed high reproducibility for a single technologist and between technologists, with a kappa agreement of 0.77. The within-center agreements of the five in-house PCR tests varied from 0.19 to 0.74 on two challenges of 60 specimens 1 month apart. The LCx C. pneumoniae RUO PCR shows excellent potential for use in clinical studies, which could enable standardization of results in the field.
dc.language.isoenen_US
dc.relation.urlhttps://journals.asm.org/doi/10.1128/JCM.40.7.2357-2362.2002en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleComparison of an industry-derived LCx Chlamydia pneumoniae PCR research kit to in-house assays performed in five laboratories.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleJournal of clinical microbiologyen_US
dc.source.volume40
dc.source.issue7
dc.source.beginpage2357
dc.source.endpage62
dc.source.countryUnited States
dc.description.versionVoRen_US
refterms.dateFOA2023-07-05T15:56:19Z
html.description.abstractIn a multicenter comparison of PCR assays utilizing 120 quantitated samples of 16 Chlamydia pneumoniae isolates, an LCx research-use-only (RUO) PCR developed by Abbott Laboratories demonstrated 100% sensitivity on 48 samples with >1 copy of DNA per microl of specimen. The sensitivities of five in-house PCR assays ranged from 54 to 94% for the same samples. All six assays showed decreased sensitivities as the DNA copy numbers of the samples decreased. Overall, sensitivities ranged from 68% for the LCx PCR assay to 29% for one of the in-house tests. The LCx RUO PCR and three of the five in-house PCR tests reported no false positives with the 24 negative samples. Increasing the number of replicates tested increased the sensitivities of all of the assays, including the LCx PCR. The LCx RUO assay showed high reproducibility for a single technologist and between technologists, with a kappa agreement of 0.77. The within-center agreements of the five in-house PCR tests varied from 0.19 to 0.74 on two challenges of 60 specimens 1 month apart. The LCx C. pneumoniae RUO PCR shows excellent potential for use in clinical studies, which could enable standardization of results in the field.
dc.description.institutionSUNY Downstateen_US
dc.description.departmentPediatricsen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalJournal of clinical microbiology
dc.identifier.issue7en_US


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