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dc.contributor.authorMontalban, G S
dc.contributor.authorRoblin, P M
dc.contributor.authorHammerschlag, M R
dc.date.accessioned2023-06-30T16:14:05Z
dc.date.available2023-06-30T16:14:05Z
dc.date.issued1994-05
dc.identifier.citationMontalban GS, Roblin PM, Hammerschlag MR. Performance of three commercially available monoclonal reagents for confirmation of Chlamydia pneumoniae in cell culture. J Clin Microbiol. 1994 May;32(5):1406-7. doi: 10.1128/jcm.32.5.1406-1407.1994. PMID: 8051280; PMCID: PMC263716.en_US
dc.identifier.issn0095-1137
dc.identifier.pmid8051280
dc.identifier.urihttp://hdl.handle.net/20.500.12648/10335
dc.description.abstractWe evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal reagents and one conjugated genus-specific reagent. All three produced similar intensities of staining of C. pneumoniae, with some strain-to-strain variation. Methanol appeared to be a better choice of fixative than acetone, which greatly reduced the intensity of fluorescence with one of the species-specific antibodies.
dc.language.isoenen_US
dc.relation.urlhttps://journals.asm.org/doi/epdf/10.1128/jcm.32.5.1406-1407.1994en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePerformance of three commercially available monoclonal reagents for confirmation of Chlamydia pneumoniae in cell culture.en_US
dc.typeArticle/Reviewen_US
dc.source.journaltitleJournal of clinical microbiologyen_US
dc.source.volume32
dc.source.issue5
dc.source.beginpage1406
dc.source.endpage7
dc.source.countryUnited States
dc.description.versionVoRen_US
refterms.dateFOA2023-06-30T16:14:06Z
html.description.abstractWe evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal reagents and one conjugated genus-specific reagent. All three produced similar intensities of staining of C. pneumoniae, with some strain-to-strain variation. Methanol appeared to be a better choice of fixative than acetone, which greatly reduced the intensity of fluorescence with one of the species-specific antibodies.
dc.description.institutionSUNY Downstateen_US
dc.description.departmentPediatricsen_US
dc.description.degreelevelN/Aen_US
dc.identifier.journalJournal of clinical microbiology
dc.identifier.issue5en_US


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