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2021
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Quest2021_01.pdf
Adobe PDF, 239.94 KB
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Abstract
Enzyme 2O14 is a protein of unknown function, but is hypothesized to be
an esterase. We investigated the function of this enzyme using a
chromogenic ester substrate, p-nitrophenyl acetate (PNPA). The product
from the hydrolysis of PNPA is yellow, allowing for the measurement of
absorbance as a function of activity. Enzyme 2O14 was shown to
hydrolyze the ester substrate, indicated by the increase in absorbance as a
result of the formation of the yellow product.