Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.

Bandea, Claudiu I; Joseph, Kahaliah; Secor, Evan W; Jones, Laurie A; Igietseme, Joseph U; Sautter, Robert L; Hammerschlag, Margaret R; Fajman, Nancy N; Girardet, Rebecca G; Black, Carolyn M

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Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.

Bandea, Claudiu I; Joseph, Kahaliah; Secor, Evan W; Jones, Laurie A; Igietseme, Joseph U; Sautter, Robert L; Hammerschlag, Margaret R; Fajman, Nancy N; Girardet, Rebecca G; Black, Carolyn M

Journal Title

Journal of clinical microbiology

Publication Date

2013-04

Publication Volume

51

Publication Issue

4

Publication Begin

1298

Publication End

300

Abstract

Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.

Citation

Bandea CI, Joseph K, Secor EW, Jones LA, Igietseme JU, Sautter RL, Hammerschlag MR, Fajman NN, Girardet RG, Black CM. Development of PCR assays for detection of Trichomonas vaginalis in urine specimens. J Clin Microbiol. 2013 Apr;51(4):1298-300. doi: 10.1128/JCM.03101-12. Epub 2013 Feb 6. PMID: 23390274; PMCID: PMC3666790.

DOI

10.1128/JCM.03101-12

Development of PCR assays for detection of Trichomonas vaginalis in urine specimens.

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