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Performance of three commercially available monoclonal reagents for confirmation of Chlamydia pneumoniae in cell culture.
Montalban, G S Roblin, P M Hammerschlag, M R
Montalban, G S
Roblin, P M
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Journal of clinical microbiology
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1994-05
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Publication Volume
32
Publication Issue
5
Publication Begin
1406
Publication End
7
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Abstract
We evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal reagents and one conjugated genus-specific reagent. All three produced similar intensities of staining of C. pneumoniae, with some strain-to-strain variation. Methanol appeared to be a better choice of fixative than acetone, which greatly reduced the intensity of fluorescence with one of the species-specific antibodies.
Citation
Montalban GS, Roblin PM, Hammerschlag MR. Performance of three commercially available monoclonal reagents for confirmation of Chlamydia pneumoniae in cell culture. J Clin Microbiol. 1994 May;32(5):1406-7. doi: 10.1128/jcm.32.5.1406-1407.1994. PMID: 8051280; PMCID: PMC263716.