Loading...
Anthropomorphizing the Mouse Cardiac Action Potential via a Novel Dynamic Clamp Method
Journal Title
Biophysical Journal
Keywords
Readers/Advisors
Journal Title
Term and Year
Publication Date
2009-11
Book Title
Publication Volume
97
Publication Issue
10
Publication Begin
2684
Publication End
2692
Number of pages
Collections
Files
Research Projects
Organizational Units
Journal Issue
Abstract
Interspecies differences can limit the translational value of excitable cells isolated from model organisms. It can be difficult to extrapolate from a drug- or mutation-induced phenotype in mice to human pathophysiology because mouse and human cardiac electrodynamics differ greatly. We present a hybrid computational-experimental technique, the cell-type transforming clamp, which is designed to overcome such differences by using a calculated compensatory current to convert the macroscopic electrical behavior of an isolated cell into that of a different cell type. We demonstrate the technique's utility by evaluating drug arrhythmogenicity in murine cardiomyocytes that are transformed to behave like human myocytes. Whereas we use the cell-type transforming clamp in this work to convert between mouse and human electrodynamics, the technique could be adapted to convert between the action potential morphologies of any two cell types of interest.
Citation
Ahrens-Nicklas RC, Christini DJ. Anthropomorphizing the mouse cardiac action potential via a novel dynamic clamp method. Biophys J. 2009 Nov 18;97(10):2684-92. doi: 10.1016/j.bpj.2009.09.002. PMID: 19917221; PMCID: PMC2776258.