Loading...
Overexpression of Map3k7 activates sinoatrial node-like differentiation in mouse ES-derived cardiomyocytes
Brown, Kemar Legros, Stephanie Ortega, Francis A. Dai, Yunkai Doss, Michael Xavier Christini, David J. Robinson, Richard B. Foley, Ann C.
Brown, Kemar
Legros, Stephanie
Ortega, Francis A.
Dai, Yunkai
Doss, Michael Xavier
Christini, David J.
Robinson, Richard B.
Foley, Ann C.
Citations
Altmetric:
Journal Title
PLOS ONE
Keywords
Readers/Advisors
Journal Title
Term and Year
Publication Date
2017-12-27
Type
Book Title
Publication Volume
12
Publication Issue
12
Publication Begin
e0189818
Publication End
Number of pages
Collections
Files
Loading...
file (9).pdf
Adobe PDF, 10.8 MB
Research Projects
Organizational Units
Journal Issue
Abstract
In vivo, cardiomyocytes comprise a heterogeneous population of contractile cells defined by unique electrophysiologies, molecular markers and morphologies. The mechanisms directing myocardial cells to specific sub-lineages remain poorly understood. Here we report that overexpression of TGFβ-Activated Kinase (TAK1/Map3k7) in mouse embryonic stem (ES) cells faithfully directs myocardial differentiation of embryoid body (EB)-derived cardiac cells toward the sinoatrial node (SAN) lineage. Most cardiac cells in Map3k7-overexpressing EBs adopt markers, cellular morphologies, and electrophysiological behaviors characteristic of the SAN. These data, in addition to the fact that Map3k7 is upregulated in the sinus venous-the source of cells for the SAN-suggest that Map3k7 may be an endogenous regulator of the SAN fate.
Citation
Brown K, Legros S, Ortega FA, Dai Y, Doss MX, Christini DJ, Robinson RB, Foley AC. Overexpression of Map3k7 activates sinoatrial node-like differentiation in mouse ES-derived cardiomyocytes. PLoS One. 2017 Dec 27;12(12):e0189818. doi: 10.1371/journal.pone.0189818. PMID: 29281682; PMCID: PMC5744947.