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Jiang, Xian-Cheng
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Fall 2015
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2015-08-10
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Master's Thesis
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Abstract
The sphingomyelin synthase (SMS) family has three members. The two sphingomyelin synthase isoforms, SMS1 and SMS2, have been well studied, but the most conservative member, sphingomyelin synthase related protein (SMSr) has still not been well characterized. It has been reported that SMSr is
able to synthesize ceramide phosphoethanolamine (CPE). However, knockout of SMSr gene in mice did
not significantly influence CPE levels which are extremely low in the tissues. Moreover, both SMS1
and SMS2 have similar CPE synthase activity. Thus, the major biological function or activity of
SMSr is still unknown.
Based on SMSr active site motif, I hypothesized that 1) SMSr has ceramide-1-phosphate phosphatase
activity; and 2) SMSr has phosphatidylcholine-specific phospholipase C activity. In this project, I
have a specific aim to test these two hypotheses. I found that, under my established conditions,
SMSr has neither C1P phosphatase activity nor PC-PLC activity. I discussed the limitation of my conclusion and proposed the future plan for the project.
Citation
Zhao, Wan (2015), The potential function of sphingomyelin synthase related protein (SMSr) Master's thesis dissertation, SUNY Downstate Health Sciences University]. SUNY Open Access Repository. https://soar.suny.edu/handle/20.500.12648/17050